OHSU

Sample Amplification and Labeling

Labeling Requirements and Pricing

Sample Amplification and Labeling Options for Microarray Expression Profiling

Notes: 

  • If the concentration of samples is unknown, please contact the Gene Microarray Shared Resource lab before submitting samples.
  • When submitting samples, RNA should be in an RNAse, DNAse-free 1.5ml microcentrifuge tube and in aqueous solution, preferably nuclease-free water.
  • When submitting samples with small RNA inputs (5-100ng total), please contact the Gene Microarray Shared Resource lab before submitting samples for any special considerations.
  • For any sample preparation and sample submission questions, contact the Gene Microarray Shared Resource lab.Reduced pricing is available for batch processing of 24 or more samples.
  • To submit polyA+ RNA for expression profiling, please contact the Gene Microarray Shared Resource lab for submission requirements.

RNA profiling

3'IVT Labeling method*
 Sample type
Total RNA submission requirement** (minimum)

 (ng)
Minimum
concentration

(ng/µl)
Total RNA input required for target labeling

(ng)

 Price per sample
Ambion MessageAMP Premier (Standard) total RNA
 600 40


200

 $210
Affymetrix One Cycle cDNA synthesis
 total RNA
 3000 200


2000

 $210
NuGEN Ovation Labeling
 total RNA
 200 10


50

 $290
Whole Transcript     Labeling for Gene and Exon arrays*
 Sample type
Total RNA submission requirement** (minimum)

 (ng)
Minimum

concentration
(ng/µl)

Total RNA input required for target labeling (ng)

 Price per sample
Ambion Whole Transcript Sense target labeling (Standard)
 total RNA
 600 20

100
 $150

miRNA labeling

for Affymetrix

miRNA array*
Sample

type
Total RNA
submission
requirement** (minimum)

 (ng)
Minimum

concentration
(ng/µl)

RNA (total/LMW enriched)

input required for

target labeling (ng)

 Price per sample

Genisphere High Sensitivity

FlashTag Biotin labeling

total RNA 

(which contains LMW RNA)

or

low molecular weight (LMW) enriched RNA

 600

12.5 (if isolated from tissue)

25 (if isolated from cultured cells)



100

(if isolated from tissue;

input sample may be

(1) total RNA,

which contains low

molecular weight (LMW) RNA

or (2) LMW enriched RNA)

 

200 

(if input is LMW enriched 

RNA only 

and sample was isolated

from cultured cells)

 

500

(if input is total RNA,

which contains

LMW RNA,

and sample

was isolated from

cultured cells)

 $90

** Includes sufficient material for RNA quantification, quality assessment, and target labeling. Volume remaining after processing can be retrieved.

Sample Amplification and Labeling Options for Microarray DNA Variation Analysis

DNA Variation (SNP) arrays:  

DNA Labeling Method
 Sample type

DNA submission requirement** (minimum)

(ng)
Minimum

concentration (ng/µl)
DNA input required for
labeling

(ng)

 Price per sample
Affymetrix 250K
 genomic DNA
 1000 50 500 $210
Affymetrix SNP 6.0 (Standard)
 genomic DNA
 1000 50 500 $210
** Includes sufficient material for DNA quantification, quality assessment, and target labeling.

 *If you are interested in an alternate labeling method due to sample quantity limitations or other issues, please contact the Core Director.  There are no guarantees that non-standard protocols will be implemented in the core.  If a needed protocol fits into this category, the investigator's lab may consider performing the amplification and labeling in the investigator's lab and submitting the microarray target cRNA or cDNA (material ready for hybridization) to the core for hybridization, scanning, data preparation, data quality assessment, and data management.