FAQ
What are the sample quantity requirements for the various array assays?
Sample quantity requirements vary by assay and amounts indicated include sufficient material for RNA quantification and quality assessment, as well as the amount needed for the target labeling protocol.
In general, sample concentration requirements are ~200ng/μl for standard labeling method options and ~10ng/μl for small sample labeling method options.
If your sample quantity/concentration does not meet the standard minimum requirements, please contact the Affymetrix Microarray Core lab for considerations.
Affymetrix 3'IVT Expression array assays
Affymetrix Whole Transcript (WT) Expression array assays
Affymetrix DNA Variation (SNP) array assays
In general, sample concentration requirements are ~200ng/μl for standard labeling method options and ~10ng/μl for small sample labeling method options.
If your sample quantity/concentration does not meet the standard minimum requirements, please contact the Affymetrix Microarray Core lab for considerations.
For Microarray Expression Profiling assays:
Affymetrix 3'IVT Expression array assays
| 3' IVT Labeling Method |
Sample Type |
RNA submission requirement (minimum) |
| Affymetrix one cycle (Standard) |
total RNA/polyA+ purified |
1500ng |
| MessageAMP Premier (Invitrogen) | total RNA/polyA+ purified | 600ng |
| Affymetrix two cycle (small sample) | total RNA/polyA+ purified | 200ng |
| Ovation Labeling (NuGEN) | total RNA/polyA+ purified | 200ng |
Affymetrix Whole Transcript (WT) Expression array assays
| Whole Transcript Labeling Method | Sample Type | RNA submission requirement (minimum) |
| Affymetrix exon sense w/ rRNA reduction (Standard) | total RNA/polyA+ purified | 1500ng |
| Affymetrix exon sense w/o rRNA reduction | total RNA/polyA+ purified | 600ng |
| Affymetrix gene sense w/ rRNA reduction (Standard) | total RNA/polyA+ purified | 1500ng |
| Affymetrix gene sense w/o rRNA reduction | total RNA/polyA+ purified | TBA |
For Microarray DNA Variation analysis assays:
Affymetrix DNA Variation (SNP) array assays
| DNA Labeling Method | Sample type | DNA submission requirement (minimum) |
| Affymetrix 250K | genomic DNA | TBA |
| Affymetrix SNP 6.0 (Standard) | genomic DNA | TBA |
