Nucleic Acid Isolation

The GPSR provides nucleic acid extraction services for a large variety of sample types, including saliva, blood, FFPE specimens, fresh/frozen tissue and cultured cells. We specialize in Qiagen based isolation methods but also utilize other methods, such as TRIzol Reagent and Zymo Research based kits, depending on project needs.

Many of the core-supported isolation procedures can be run on Qiagen automation robots available in the lab. Available platforms: 2 QIAcubes (up to 12 samples per processing run) and a QIAsymphony (24-96 samples per processing run).  For multiple samples, automation-based processing provides an additional level of reproducibility while eliminating potential operator-to-operator variation. 

The GPSR also provides support for nucleic acid sample processing to remove contaminants from RNA and DNA solutions and to concentrate samples when required.

QIAcube              QIAsymphony              Qiagen TissueLyser LT

QIAcube Isolation Robot                                                                    QIAsymphony Isolation Robot                    QIAGEN TissueLyser LT
Automated RNA/DNA Isolation using                                               High-throughput automated                          Provides fast, effective disruption of    
Qiagen column based kits                                                                     RNA/DNA isolation                                              up to 12 samples at the same time

  • DNA (fresh, frozen, FFPE)
    • GPSR low throughput DNA isolation service (1-24 samples), includes standard Quality Assessment service (UV quantification and Tapestation sizing).
    • GPSR high throughput automated DNA isolation service from cells and tissue (24+ samples) is available at low, per sample cost, includes UV quantification. 
    • GPSR high throughput automated DNA isolation service from 2ml Saliva (24+ samples) is available at low, per sample cost, includes UV quantification. 
  • RNA (fresh, frozen, FFPE, prokaryotic, eukaryotic) quality assessment
    • GPSR low throughput RNA isolation service (1-24 samples) includes standard Quality Assessment service (UV quantification and Bioanalyzer sizing).
    • GPSR high throughput automated RNA isolation service from cells and tissue (24+ samples) is available at low, per sample cost, includes standard Quality Assessment service (UV quantification and Bioanalyzer sizing)
    • GPSR high throughput automated RNA isolation service from PAXgene RNA blood tubes (24+ samples) is available at low, per sample cost, includes standard Quality Assessment service (UV quantification and Bioanalyzer sizing).
  • Processing for nucleic acid cleanup and/or concentration

Support for Biofluid-Specific Collection Protocols

PAXgene blood tubes Oragene saliva vials
   PAXgene blood tubes                                 Genotek saliva vials
  • The Core supports RNA and DNA Isolation protocols that use the following collection tubes:
PreAnalytiX collection tubes for blood preservation of both RNA and DNA. 
DNA Genotek collection vials for oral, fecal, blood, saliva, and sputum collection.

The Core is available to consult on optimal collection protocols.  Please contact us if we can be of assistance.

Sample Preparation and Delivery

For information about sample preparation and delivery, please see the instructions here.