Resources
Equipment
- NEW! Becton Dickinson InFlux: Can sort up to eleven fluorescent probes rapidly to purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously.
- Becton Dickinson InFlux: Can sort up to ten fluorescent probes rapidly to purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously.
- Becton Dickinson Turbo DiVa Vantage® cell sorter: Can sort up to eight different fluorescent probes at up to 20,000 cells/second and purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously.
- NEW! Miltenyi MACSQuant: three-laser system allowing for up to seven-color analysis from tubes or 96-well plates, with absolute cell counts by volume.
- NEW! Miltenyi AutoMACS Pro: magnetic bead separation allows for cell enrichment or pre-sorting prior to InFlux or Vantage use.
- BD LSR II ®: three-laser system allows for rapid, simultaneous eight-color analysis.
- BD FACSCalibur® analyzer: This two-laser instrument is capable of one- to four-color analyses along with size and cellular granularity.
PROTOCOLS
- Cell cycle analysis
- General immunofluorescence staining protocol
- Intracellular staining method
- CellQuest set up and general use
- CellQuest set up for Cell Cycle
- LSR setup and general use.
