Resources
Equipment
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NEW! DVS Sciences CyTOF®: Mass Cytometer capable of highly multiplexed, single cell analysis of up to 33+ metal labeled antibodies, without the need for compensation.
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Two Becton Dickinson InFlux® cell sorters: Can sort up to eleven fluorescent probes rapidly to purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously.
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Becton Dickinson Turbo DiVa Vantage® cell sorter: Can sort up to eight different fluorescent probes at up to 20,000 cells/second and purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously.
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NEW! BD Fortessa®: Four lasers and 18 parameters expands the amount of data collected from a single tube. This analyzer is optimal for experiments with limited cell numbers.
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Miltenyi MACSQuant®: three-laser system allowing for up to seven-color analysis from tubes or 96-well plates, with absolute cell counts by volume.
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Miltenyi AutoMACS Pro®: magnetic bead separation allows for cell enrichment or pre-sorting prior to InFlux or Vantage use.
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BD LSR II®: three-laser system allows for rapid, simultaneous eight-color analysis.
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NEW! BD Canto II®: 5-color system with a UV laser for Hoechst/DAPI viability or cell cycle analysis.
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BD FACSCalibur®: This two-laser instrument is capable of one- to four-color analyses along with size and cellular granularity.
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NEW! Luminex 200®: For genomic and proteomic assays including protein expression, cell signaling, genotyping and metabolic markers. Multiplex up to 100 analytes in one microplate well.
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NEW! Becman Coulter Vi-Cell®: Fast and cost-effective cell counter adds trypan to samples and generates images and data representing cell size distribution, concentration and viability.
PROTOCOLS
- Cell cycle analysis
- General immunofluorescence staining protocol
- Intracellular staining method
- CellQuest set up and general use
- CellQuest set up for Cell Cycle
- LSR setup and general use.
