Work Request

Billing Information

We perform long-read (Sanger) DNA sequencing & genomic analysis.  Additionally, we facilitate ordering oligonucleotide (DNA, RNA, siRNA) synthesis with outside service providers.

For DNA sequencing, please login to iLab and submit a service request. Then Deliver your samples to us Using one of the following options: bring your samples to our Marquam Hill lab (RJH 5330), drop them off at our all-hours Marquam Hill location (fridge outside RJH 5341) or place your samples in our South Waterfront sample drop-off fridge in the Collaborative Life Sciences Building for daily pickup. For detailed instructions regarding our South Waterfront sample drop off please navigate to our Contact Us page.

For Oligo synthesis, please fill out this form: OSYN Work Request

If you have a large oligo synthesis order you may find the batch order form (below) easier to use.

DNA Sequencing Work-request Information

We will need the following information:
  • Service type: Pick the level of service you need. More information
  • Type of DNA template: Pick a template type, where "large" plasmids are >10 Kb, cosmids and BACS are >30 kb, small PCR products are <300 bp, large PCR products are >1000 bp. High GC templates have >60% GC content.
  • Template Purification Method: Let us know what vendor/product you used to purify your DNA template. This helps us with any troubleshooting we might need to do.

Oligo Synthesis Work-Request Information

We will need the following information:
  • Synthesis source: Choose one of our outside source providers.
  • Synthesis type: Choose either DNA (deoxy-oligo up to 150 nts long), RNA , or special. If the latter, please explain in the comment field.
  • Scale: Pick the scale of synthesis where 40 nmole is primer scale and more than enough for most applications , 200 nmole is for hybridization probes or other apps using tons of oligo, and 1 µmole if for physical studies and such.
  • Post-Synthesis Process: Simple desalting with a Nap-10 Sephadex column gives the highest yield and is recommended for most applications. Reverse-phase cartridge purification is selected if greater purity is required. We no longer offer PAGE purification as a service. Contact if you think you need this.
  • Enter Name and Sequence: There is room for up to ten (10) oligos on each work-request. If you need more, you might find using the batch order spreadsheet more convenient.
  • Comment: If the oligo is to be labeled, contains modified bases, or is unusual in other ways, include this info in the comment box. Example: a 5' biotinylated oligo would look something like: 5GATCGACT and the 5 would be defined as 5'-biotin in the comment box.