OHSU

DSEQ Work Request

Capillary "Long read" Sequence Analysis

Capillary, fluorescent, Sanger cycle sequencing

We perform DNA sequencing & fragment analysis with our state-of-the-art, ABI 3130xl, capillary fluorescence instrument. Several levels of service are available to match your research needs and finances. We have a "break-even" fee structure, i.e., charge what it costs to provide the service.

(New clients: please read this!)
 Notes on Template Purification

For lots of samples, the batch order spreadsheet might be easier to use.

Three Sample drop-off refrigerators: Hiltop: RJH 5330 and RJH 5340; Waterfront: CHH, Room 13160, and at PSU in Room 31 B2, SRTC bldg 2 (J Singer lab)

Please Note: We have installed a 48 capillary DNA sequencer, funded by our NIH shared instrumentation grant. The upgrade will triple our throughput, which means faster turnaround time for you. Please send me feedback on data quality, length of read, and turnaround time.


Last name of the Grant account PI.

For OHSU accounts: use your FAID for the account, format is 4 CAP LETTERS, 4 digits. For non-OHSU accounts: enter your email username

This is the email address we will use to notify you when ready for pick-up.

   
   
   
   
   
A = Cadillac, B = Hybrid, C = Scooter (economical), D = Bicycle (DIY), E = Fragment Analysis
Use 'Type' and 'Subtype' to customize cycle sequencing conditions.

Example: Exo/Sap or Qiagen tip-20, or QiaSpin, Promega Wizard SV, etc

Number of samples to run on the sequencer (note: one template in both directions is 2 runs).

Separate each name with a comma, no more than 13 characters per name.  Do not use these characters: \, /,:,*,?,",<,>,|   Also, do not use line-feeds or returns. Thanks!

Only needed for A and B mode samples.

Edit this field for special instructions and comments.
 
 

Required fields are bold. Press "Submit", then check your entries. Use "Edit" to make changes.  Print a copy for your records. Finally, (whew!) press "Confirm" to send us the data as an email. Remember:
Print the page first, then Confirm to send the work-request. 

Then bring the samples to the core lab. An "after hours" frig is available too.

If you have lots of DNA sequencing samples, a spreadsheet may be easier to use: DSEQ Batch Order