OHSU

Sequencing Primers

Good sequencing requires uniquely binding primers

Use high-quality sequence data to design your sequencing (and PCR) primers. An on-line tool is available at the MIT website.


House Primers

Free to our DNA Sequencing Clients

Core Lab "Common" Primers
Primer name Sequence (5' to 3')
M13/pUC(-21) F TGT AAA ACG ACG GCC ATG
M13/pUC RP CAG GAA ACA GCT ATG ACC
SP6 TAT TTA GGT GAC ACT ATA G
T3 ATT AAC CCT CAC TAA AGG GA
T7 promoter TAA TAC GAC TCA CTA TAG GG
T7 terminator GCT AGT TAT TGC TCA GCG G
pGEX-5' GGG CTG GCA AGC CAC TGG TGG TG
pGEX-3' CCG GGA GCT GCA TGT GTC AGA GG
pQE9-F115 TAT GAG AGG ATC GCA TCA CCA T
pQE9-R348 TTG GGA TAT ATC AAC GGT GGT A

If you design your own primer, be sure to use data of known, high quality, i.e., phred-value >30.
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