Sequencing Primers
Good sequencing requires uniquely binding primers
Use high-quality sequence data to design your sequencing (and PCR) primers. An on-line tool is available at the MIT website.
House Primers
Free to our DNA Sequencing Clients
| Primer name | Sequence (5' to 3') |
|---|---|
| M13/pUC(-21) F | TGT AAA ACG ACG GCC ATG |
| M13/pUC RP | CAG GAA ACA GCT ATG ACC |
| SP6 | TAT TTA GGT GAC ACT ATA G |
| T3 | ATT AAC CCT CAC TAA AGG GA |
| T7 promoter | TAA TAC GAC TCA CTA TAG GG |
| T7 terminator | GCT AGT TAT TGC TCA GCG G |
| pGEX-5' | GGG CTG GCA AGC CAC TGG TGG TG |
| pGEX-3' | CCG GGA GCT GCA TGT GTC AGA GG |
| pQE9-F115 | TAT GAG AGG ATC GCA TCA CCA T |
| pQE9-R348 | TTG GGA TAT ATC AAC GGT GGT A |
If you design your own primer, be sure to use data of known, high quality, i.e., phred-value >30.
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