Long-read DNA Sequencing, Fragment Analysis and Human Cell Line Authentication
We use an Applied Biosystems 48-capillary 3730xl automated sequence analysis system purchased through our NIH-NCRR shared instrumentation grant (S10-RR020977). It has a fast laser and uses the "POP-7®" multi-use, high-resolution polymer for fast fluorescent data collection. The system provides automated polymer loading, sample loading, detection and analysis of both DNA sequencing, fragment analysis and cell line authentication samples. Ancillary equipment for cycle sequencing is formatted for 96-well plates.
We are using the Applied Biosystems "Sequence Analysis Software" v.5.2 with the "KB" basecaller v.1.2
Salts and EDTA can interfere with polymerase activity. Choose a template purification protocol specifically for fluorescent sequencing; attention to detail is critical.
We can perform data assembly and analysis for large sequencing and fragment analysis (SNP, AFLP, etc.). Call or email regarding help with bioinformatics and research database development.