Troubleshooting
My labeling bleaches during imaging. What do I do?
- Mount your specimen in a medium that includes antifade reagents.
- Change your imaging parameters to use less excitation light for shorter periods of time.
My cells die during imaging. How can I prevent harming cells during the imaging?
- Reduce the frequency of taking images.
- Reduce excitation intensity and duration.
- Increase labeling intensity.
