Zeiss Elyra PS.1
The Zeiss Elyra PS.1 is a combination setup for the following imaging modalities -
- Laser-scanning confocal microscopy (LSM710). Pinhole-based out-of-focus light rejection in confocal microscopy has long been used for high-resolution imaging. On our setup, this well-accepted methodology can be compared to the more advanced super-resolution technologies without moving the sample.
Super-resolution structured illumination microscopy or SIM
Sim is an easily accessible super-resolution technology that uses standard fluorophores and yields up to 8-fold improvement in voxel resolution.
- Single-molecule localization microscopy, such as PALM and STORM.
Single molecule localization techniques generally require use of unusual labeling techniques, but can yield more than ten-fold lateral resolution improvement. Note that imaging is limited to area close to the coverslip.
Lasers (SR / LSM and Airyscan)
405nm / 405nm
488nm / 488nm (Argon)
561nm / 561nm
642nm / 633nm
- 10x0.3 PlanNeofluar
- 25x0.8 PlanNeofluar
- 63x1.4 PlanApo
- 100x1.46 alpha PlanApo