Mandel Lab

Senior Scientist and Principal Investigator: Gail Mandel

Research in my lab is focused on understanding how neuronal cell identity is established and maintained. Unexpectedly, we discovered that this is achieved primarily through a repressor mechanism. At the heart of this mechanism lies the DNA-binding protein, REST. Together with its interaction partners, REST controls the epigenetic status of neuronal gene chromatin. Knowledge of this mechanism provides a window into the molecular events governing nervous system formation. We are exploiting embryonic stem cell differentiation and knock out animals to understand how the repressor complex is regulated during normal development. Using a novel strategy for characterizing transcription factor binding sites, we have elucidated the REST genetic network and are studying how this network, and its associated chromatin signature, directs normal neuronal differentiation and contributes to autism spectrum disorders. Most recently, we have extended our studies to explore neuronal:glial interactions. We have uncovered a potential role for glia in inducing neuronal dysfunction in Rett Syndrome, one of the most common causes of mental retardation in young girls. Our goal is to identify how the glial genes or proteins cause the underlying neuronal pathology.

Current Lab Members

Saurabh Garg
Research Assistant Scientist
Email: garg@ohsu.edu

Susan Kim
Research Assistant II
Email: ksus@ohsu.edu

Michael Linhoff
Research Assistant Scientist
E-mail: linhoffm@ohsu.edu

James McGann
Postdoctoral Fellow
Email: mcgann@ohsu.edu

Justine Nguyen
Research Assistant II
Email: nguyjust@ohsu.edu

Benjamin Rakela
Graduate Student
E-mail: rakela@ohsu.edu

John Sinnamon
Postdoctoral Fellow
E-mail: sinnamon@ohsu.edu