Core Services
The DNA Sequencing and Tissue Culture support cores provide specialized and widely utilized scientific procedures and services which facilitate research projects in the Vollum. They operate on a fee-for-service basis with charges designed to recover the cost of staff time and materials. An Illustrations core is available for assistance with manuscripts, slides, and posters.
Automated DNA Sequencing Core
The Vollum DNA Sequencing Core provides DNA sequencing services to the scientists at the Vollum Institute and to other research units and departments at OHSU on a fee-for-service basis.
We are currently using an ABI 3130XL sequencer, which is capable of running 174 samples in 24 hours. Samples may be dropped off at any time, but each day's runs are picked up at 10:00 am. General turn-around time is 1–2 days, when you will receive e-mail notification and your data will be made available online.
Using the Sequencing Core
Prepared samples may be left in our drop off refrigerator on the 4th floor of the Vollum. Please see detailed instructions in our handy User's Guide (PDF) below. The Vollum DNA Sequencing Core Request Form is available for both Vollum and non-Vollum users as a PDF form. Right-click (PC) or control-click (Mac) to download, or click to open the file in the browser window using Adobe Acrobat. The PDF can be filled out electronically using Acrobat or Acrobat Reader, or it may be printed and filled out by hand. Submit a printed copy of the completed form when dropping off your samples.
Contact information
Phone: 503-494-7922
E-mail: dnaseq@ohsu.edu
Vollum DNA Sequencing Core User's Guide (44 KB)
Vollum DNA Sequencing Core Request Form (108 KB)
Download Adobe Acrobat Reader software
Tissue Culture Core
The Tissue Culture Core stocks commonly used items such as media, supplements and transfection reagents for many standard mammalian and fly cell culturing protocols. Items are available for after-hours purchase and in conveniently sized aliquots. Large scale purchases ensure lot consistency for greater experimental consistency and cost savings. Contact the core manager for tissue culture advice and special orders for media or if an incubator or hood requires maintenance or cleaning.
Contact information
Phone: 503-494-2470
Email: vollumtc@ohsu.edu
