Imaging and Morphology Support Core
The Imaging and Morphology Support Core of the ONPRC is designed to meet the imaging needs of ONPRC scientists and of the OHSU scientific community by providing state-of-the-art facilities, expertise, assistance and training in the use of advanced light microscopy, image analysis, processing and printing, and laser capture microdissection. Special emphasis is placed on quantitative evaluation of imaging experiments using both stereology and automated image analysis.
SERVICES
Consultation, training and access to instruments or full support by core personnel are offered for all services. The core comprises two units:Imaging Facility (contact Anda Cornea)
- Fluorescence imaging: confocal, wide-field, and deconvolution microscopy
- Special fluorescence techniques: FURA-2, FRET, FRAP, and live-cell time-lapse imaging
- Large-area image montages: color brightfield or fluorescence, including 3D confocal
- Image processing, 3D rendering, movies
- Quantitative image analysis and stereology
- Laser capture microdissection
EQUIPMENT
Imaging Facility (contact Anda Cornea)
- Leica SP5 AOBS spectral confocal system with 5 excitation lasers (405, 458, 476, 488, 514, 561, 594, 633nm), motorized stage and stage-top microincubator.
- 2-photon imaging system (to come soon)
- Marianas Imaging Workstation with motorized stage, DG4 fluorescence illumination and the stereology, FURA and FRET packages
- ArcturusXT laser capture microdissection system with UV cutting and fluorescence capability.
- Neurolucida and StereInvestigator system equipped with fluorescence.
- Image J, Volocity, Slidebook, and NewCAST
ACCESS
The Imaging and Medical Illustrations Support Core (IMSC) is a fee-for-service facility open to all ONPRC and OHSU investigators, and to the scientific community at large. Trained users may schedule independent instrument time on a web-based calendar. Core personnel are open to scientific collaborations. IMSC is located within ONPRC, on OHSU's West Campus.
