Widefield Fluorescence Microscope

Leica DMIRBE inverted microscope equipped for phase, DIC and fluorescence microscopy

• Objectives

  • 2.5x/0.07N Plan
  • 10x/0.25 PH1 N Plan
  • 20x/0.50 PH1 Plan Fluotar
  • 40x/0.55 Corr PH2 N Plan
  • 63x/0.70 Corr PH2 Plan Fluotar
  • 16x/0.50 Oil PH2 Plan Fluotar
  • 40x/1.00 Oil PH3 Fluotar
  • 63x/1.32 Oil PH3 Plan Apo

  • 100x/1.30 Oil Plan Fluotar

• Filter sets for specific detection of

  • DAPI/Hoechst
  • CFP
  • GFP, FITC, A488
  • YFP
  • RFP, Cy3

  • Cy5, Alexa633, Alexa647

• Heated live imaging chamber for 18mm round coverslips with optional perfusion system with an in-line solution heater

• X, Y and Z stage control with software-based autofocusing

• Illumination control including shutters, light attenuation and fluorescence filter switcher

MicroMax 12bit/5MHz CCD camera (Roper Scientific) with Sony 1300x1030 interline chip

MetaMorph Image Acquisition and Analysis software V. 7.5 (PC-based)

Optional rapid excitation wavelength switcher for sequential CFP/YFP  or GFP/RFP acquisition with minimal (<3ms) time delay (Xe illumination)