Microscopy
CROET has an outstanding physical facility for handling samples for light or electron microscopy. We have a unique multi-suite perfusion, dissection, and embedding area. This area is fully equipped with two down draft autopsy tables; five fume hoods, two walk in refrigerators, a walk in freezer, surgical instruments, and vapor anesthetic machines. Tissues of interest may be prepared using techniques from fresh frozen for fluorescence microscopy to embedding in plastic for electron microscopy. Samples may be sectioned using a cryostat, paraffin microtome, or ultramicrotome. Samples may be viewed in phase contrast, differential interference contrast, bright field, darkfield, fluorescence, and transmission electron microscopy.
Usage Fees
Histology has a basic Buy-in-fee to cover supplies and service contracts ($383.94/fiscal year, July 1 to June 30). If a principal investigator pays the fee, he and his staff will have full access to the facility, equipment, and a reasonable and equitable share of the supplies. Core users are expected to sign up as needed and share the facilities and supplies.
Work can be done by the core and will be charged out at $34.31/hour.
Use of the electron microscope is also charged out at $34.31/hour.
Sample Preparation (By Special Arrangement)
Light and Electron Microscopy
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Fix
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Embedding
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Sectioning
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Staining
