OHSU

Laser Scanning Confocal Microscope

  • Olympus FluoView 300 Confocal mounted on a BX51 upright microscope equipped for fluorescence and DIC

  • Laser lines at

    • 488nm [Argon] for e.g. GFP, FITC

    • 543nm [HeNe Green] for e.g. Rhodamine, Cy3, Alexa546, Alexa555

    • 633nm [HeNe Red] for e.g. Cy5, Alexa633, Alexa647

  • Confocal transmitted light detection

  • Objectives

    • 10x/0.3 U Plan Fluotar

    • 20x/0.7 U Plan Apo

    • 40x/1.35 Corr U Apo Oil Imm

    • 60x/1.4 Plan Apo Oil Imm

    • 60x/1.2 U Plan Apo Water Imm

    • 100x/1.35 Corr U Plan Apo Oil Imm