OHSU

Faculty & Staff

Stefanie Kaech Petrie, Ph.D.

Email:
kaechs@ohsu.edu
Position:
Associate Professor, Neurology
Director, Advanced Light Microscopy Core
Office:
BRB 405

Research Interests

The first time I watched a time-lapse recording of a living cell I became fascinated by the power of digital microscopy.  The discovery of GFP and its application to visualize dynamic events inside of cells has revolutionized modern cell biology.  I first used this enabling technology to study the structural proteins that give a neuron its shape and moved on to study membrane protein trafficking when I arrived in Portland. In my current role as the Director of the Advanced Light Microscopy Core I get to discuss imaging projects with students, postdoc and faculty, teach them the use of the high-end equipment in the Core, and share their excitement when the images obtained are revealing for their research. 

Biography

Dr. Kaech Petrie obtained her Ph.D. in Cell Biology in 1991 at the University of Basel, Switzerland. After a short post-doctoral experience at NINDS-NIH in Bethesda, Maryland, she joined the laboratory of Dr. Andrew Matus at the Friedrich Miescher Institute in Basel, Switzerland, where she specialized in visualization of dynamic processes in living cells using GFP, a technology just emerging at that time.  She joined Dr. Gary Banker's laboratory at OHSU in 2000 and transitioned to become the Director of the Advanced Light Microscopy Core @ The Jungers Center in 2009.

Video

Links

Selected Publications

Kaech, S, Huang, CF, and Banker, G (2012) General considerations for live imaging of developing hippocampal neurons in culture.  Cold Spring Harb Protoc 2012(3):312-8. Abstract
 
Kaech, S, Huang, CF, and Banker, G. (2012) Long-term time-lapse imaging of developing hippocampal neurons in culture.  Cold Spring Harb Protoc 2012(3):335-9. Abstract
 
Kaech, S, Huang, CF, and Banker, G (2012) Short-term high-resolution imaging of developing hippocampal neurons in culture.  Cold Spring Harb Protoc 2012(3):340-3. Abstract

Liu, C, Takahashi M, Li Y, Dillon TJ, Kaech S, and Stork, PJS  (2010) The interaction of Epac1 and Ran promotes Rap1 activation at the nuclear envelope.  Mol Cell Biol. 30:3956-69. Abstract

Kaech S, Banker G (2007) Culturing hippocampal neurons. Nat Protoc 1:2406-15. Abstract

Sampo, B, Kaech, S. Kunz, S, and Banker, G  (2003)  Two Distinct Mechanisms Target Membrane Proteins to the Axonal Surface.  Neuron 37:611-624. Abstract

Fischer M, Kaech S, Knutti D, Matus A. (1998)  Rapid actin-based plasticity in dendritic spines.  Neuron 20:847-54. Abstract

Kaech S, Ludin B, Matus A  (1996)  Cytoskeletal plasticity in cells expressing neuronal microtubule-associated proteins.  Neuron 17:1189-99. Abstract