R. Stephen Lloyd, PhD
- Email:
- lloydst@ohsu.edu
- Position:
- Professor
- Senior Scientist
Read more about Dr. Lloyd's research
Research Interests
DNA repair processes and high fidelity DNA replication represent the major mechanisms to maintain genomic stability. Our laboratory uses multi-disciplinary approaches to focus on:
- Strategies to prevent sunlight-induced skin cancer via the topical introduction of repair enzymes into human cells;
- The mechanisms by which the loss of a DNA repair enzyme can lead to the clinical manifestations of Metabolic Syndrome - a constellation of diseases (obesity, fatty liver disease, insulin resistance and hypertension) that affect >45 million Americans;
- The molecular mechanisms by which environmental toxicants create mutations in DNA; and
- The cellular response mechanisms for the repair of DNA-protein crosslinks.
Biography
Dr. R. Stephen Lloyd received his BS in Biology from Florida State University in 1975, majoring in marine pollution biology. His research interests turned to cancer chemotherapy and in 1979, he earned his Ph.D. in Molecular Biology from the University of Texas Graduate School of Biomedical Sciences in Houston, TX. After learning about mechanisms by which DNA can be damaged, he began his career in DNA repair as a postdoctoral fellow at Stanford University in the laboratory of Dr. Philip Hanawalt. Following two years at Stanford, he worked for two more years for a genetic engineering company, before joining the Biochemistry Department at Vanderbilt University in 1983. In his ten-year stay at Vanderbilt, Dr. Lloyd rose through the ranks to Full Professor, and his research focused on both DNA repair and molecular mutagenesis. He was then recruited to the Center for Molecular Science at the University of Texas Medical Branch in which the faculty exclusively specialized in DNA repair mechanisms. During his twelve years at UTMB, he also became the Director of two Centers in Environmental Toxicology. In August 2003, he, along with his wife, Dr. Amanda K. McCullough was recruited to join the CROET faculty at OHSU. Together they have both separate and joint research projects in the research areas described below.
Video
Links
Selected Publications
Fernandes PH, Kanuri M, Nechev LV, Harris TM, Lloyd RS. Related Articles, Links Mammalian cell mutagenesis of the DNA adducts of vinyl chloride and crotonaldehyde. Environ Mol Mutagen. 45: 455-9, 2005. Abstract
Minko IG, Kurtz AJ, Croteau DL, Van Houten B, Harris TM, Lloyd RS. Initiation of repair of DNA- polypeptide cross-links by the UvrABC nuclease. Biochemistry. 44: 3000-3009, 2005. Abstract
Lloyd RS. Investigations of pyrimidine dimer glycosylases--a paradigm for DNA base excision repair enzymology. Mutat Res. 577: 77-91, 2005. Abstract
Kanuri M, Nechev LV, Kiehna SE, Tamura PJ, Harris CM, Harris TM, Lloyd RS. Evidence for Escherichia coli polymerase II mutagenic bypass of intrastrand DNA crosslinks. DNA Repair (Amst). 4(12):1374-80, 2005. Abstract
Sanchez AM, Kozekov ID, Harris TM, Lloyd RS. Formation of inter- and intrastrand imine type DNA-DNA cross-links through secondary reactions of aldehydic adducts. Chem Res Toxicol. 18(11):1683-90, 2005. Abstract
Vartanian V, Lowell B, Minko IG, Wood TG, Ceci JD, George S, Ballinger SW, Corless CL, McCullough AK, Lloyd RS. The metabolic syndrome resulting from a knockout of the NEIL1 DNA glycosylase. Proc Natl Acad Sci U S A. 103(6): 1864-9, 2006. Abstract
Harbut, MB, Meador, M, Dodson, ML and Lloyd, RS. Modulation of the turnover of formamidopyrimidine DNA glycosylase. Biochem. 45(23): 7341-6, 2006. Abstract
Fernandes, PH, Hackfield, LC, Kozekov, ID, Hodge, RP, Lloyd, RS. Synthesis and mutagenesis of the butadiene-derived N3 2'-deoxyuridine adducts. Chem Res Toxicol. 19(7): 968-76, 2006. Abstract
Golan, G, Zharkov, DO, Grollman, AP, Dodson, ML, McCullough, AK, Lloyd, RS, Shoham, G. Structure of T4 pyrimidine dimer glycosylase in a reduced imine covalent complex with abasic site-containing DNA. J Molec Biol 362(2): 241-58, 2006. Abstract
