Flow Cytometry

OHSU offers a full flow cytometry service to aid in the evaluation of a variety of clinical problems such as:

  • Diagnosing and following immunodeficiency diseases, both hereditary and acquired (AIDS), using measurement of T-cell and B-cell subsets.
  • Distinguishing benign from malignant lymphoid proliferations (leukemia and lymphoma).
  • Subclassing leukemias and lymphomas into B-cell, T-cell, and mono- and myelocytic types.
  • Following immunosuppressive therapy of transplant patients with OKT3 receptor quantitation, and T-cell and B-cell subsets.
  • Diagnosing, assessing prognosis, and determining therapy in breast, bladder and other carcinomas using DNA content and cell cycle analysis.
  • Verifying immune activation in patients with symptoms of autoimmune disease, transplant rejection, chronic fatigue syndrome, overexposure to hazardous chemicals, allergy, viral infections, etc.
  • Providing miscellaneous testing for bone marrow transplant recipients, neutropenic patients, and for research and educational purposes.
  • Diagnosis of paroxysmal nocturnal hemoglobinuria.

Flow Lab test menus:

Hematologic malignancies

  • Myeloid disorders
              Acute myeloid leukemias
              Chronic myeloproliferative neoplasms
              Chronic myeloproliferative/myelodysplastic neoplasms
  • Lymphoid disorders
              Acute lymphoblastic leukemia/lymphomas
              B-cells, T-cell, and NK-cell lymphomas, all subtypes
              Large granulocytic leukemia with Kir antibodies
              Plasma cell myelomas
              Sezary syndrome

MRD (minimal residual diseases) detection (sensitivity of 0.01%)

Acute lymphoblastic leukemia
Acute myeloid leukemia
Chronic lymphocytic leukemia

Immunodeficiency and autoimmune disorders

CVID (common variable immunodeficiency)
SCID (severe combined immunodeficiency)
ALPS (autoimmune lymphoproliferative disorders)
CD4 cell count
T-cell subset count: CD3/CD4/CD8
Lymphoid subset count: T, B, and NK cells

Stem cells and bone marrow transplant services:

CD34 count
CD34 count with T-cell quant
Immune reconstitution
Sorting for Chimerism

PNH: High sensitivity assay (0.01%)

Fetal hemoglobin testing

Flow sorting

Chimerism testing
Sorting cells on slides for FISH
Sorting cells for RNA expression
Sorting cells for DNA mutation testing

The lab is open for routine testing 8am-5pm weekdays and 9am-4pm on weekends and holidays for OKT3 testing only.

Faculty members can be reached by calling 503 494-2302 during regular business hours or by calling the campus operator after hours at 503 494-8311 and asking for the Immunology faculty on-call.