OHSU offers a full flow cytometry service to aid in the evaluation of a variety of clinical problems such as:
- Diagnosing and following immunodeficiency diseases, both hereditary and acquired (AIDS), using measurement of T-cell and B-cell subsets.
- Distinguishing benign from malignant lymphoid proliferations (leukemia and lymphoma).
- Subclassing leukemias and lymphomas into B-cell, T-cell, and mono- and myelocytic types.
- Following immunosuppressive therapy of transplant patients with OKT3 receptor quantitation, and T-cell and B-cell subsets.
- Diagnosing, assessing prognosis, and determining therapy in breast, bladder and other carcinomas using DNA content and cell cycle analysis.
- Verifying immune activation in patients with symptoms of autoimmune disease, transplant rejection, chronic fatigue syndrome, overexposure to hazardous chemicals, allergy, viral infections, etc.
- Providing miscellaneous testing for bone marrow transplant recipients, neutropenic patients, and for research and educational purposes.
- Diagnosis of paroxysmal nocturnal hemoglobinuria.
Flow Lab test menus:
- Myeloid disorders
Acute myeloid leukemias
Chronic myeloproliferative neoplasms
Chronic myeloproliferative/myelodysplastic neoplasms
- Lymphoid disorders
Acute lymphoblastic leukemia/lymphomas
B-cells, T-cell, and NK-cell lymphomas, all subtypes
Large granulocytic leukemia with Kir antibodies
Plasma cell myelomas
MRD (minimal residual diseases) detection (sensitivity of 0.01%)
Acute lymphoblastic leukemia
Acute myeloid leukemia
Chronic lymphocytic leukemia
Immunodeficiency and autoimmune disorders
CVID (common variable immunodeficiency)
SCID (severe combined immunodeficiency)
ALPS (autoimmune lymphoproliferative disorders)
CD4 cell count
T-cell subset count: CD3/CD4/CD8
Lymphoid subset count: T, B, and NK cells
Stem cells and bone marrow transplant services:
CD34 count with T-cell quant
Sorting for Chimerism
PNH: High sensitivity assay (0.01%)
Platelet dense body
Fetal hemoglobin testing
The lab is open for routine testing 8am-5pm weekdays and 10am-2pm on weekends and holidays for OKT3 testing only. Arrangements can be made for after-hours services by calling 503 494-2302 during weekday business hours.
Sorting cells on slides for FISH
Sorting cells for RNA expression
Sorting cells for DNA mutation testing
Faculty members can be reached by calling 503 494-2302 during regular business hours or by calling the campus operator after hours at 503 494-8311 and asking for the Immunology faculty on-call.