Welcome to the OHSU Research Cytogenetics Core Laboratory. We are a fee-for-service cytogentics laboratory available to genetics researchers to assist in development and execution of cytogenetics experiments for research purposes. Through rigorous standardization of protocols and customized experiment development, the OHSU Research Cytogenetics Core Laboratory provides high quality metaphase and interphase cytogenetic data to the OHSU research community. Services are also available to non-profit and commercial investigators located in Oregon and elsewhere.
Services Available from the OHSU Research Cytogenetics Laboratory
- Cytogenetic experiment design and consulting
- Grant preparation assistance
- Many species available
- Rhesus Macaque
- Chinese Hamster
- Others by request
- G-Banded Karyotyping
- High resolution G-banded analysis
- Sister Chromatid Exchange
- Fluorescent In Situ Hybridization (FISH)
- Probe Development (multi-species)
- Probe design consultation
- BAC preparation
- Probe Localization
- Interphase Mapping
- Cell-Cell Fusion
- FISH following immunocytochemistry
- Ploidy assessment
- Commercial FISH probe analysis
- Others by request
- Breakage and Radial Formation
- Various DNA damaging agents
- Ploidy / Aneuploidy Assessment
- Mitotic Index
A reverse chronological listing of publications that include work by the OHSU Cytogenetics Research Laboratory.
Duncan AW, Hanlon Newell AE, Smith L, Wilson EM, Olson SB, Thayer MT, Strom SC, Grompe M. Frequent Aneuploidy Among Normal Human Hepatocytes. Gastroenterology 3 November 2011
Tian Q, Hanlon Newell AE, Wang Y, Olson S, Fedorov LM. Complex Cytogenetic Analysis of Early Lethality Mouse Embryos. Chromosome Research. In Press, 4/2011.
Duncan AW, Taylor MH, Hickey RD, Hanlon Newell AE, Lenzi ML, Olson SB, Finegold MJ, Grompe M. The ploidy conveyor of mature hepatocytes as a source of genetic variation. Nature 467 (2010) 707-710
Ramsey B, Bai T, Hanlon Newell A, Troxell M, Park B, Olson S, Keenan E, Luoh SW GRB7 protein over-expression and clinical outcome in breast cancer. Breast Cancer Res Treat. 2010 Jul 16. [Epub ahead of print]
Deininger J, Hanlon Newell A, Bumm T, Lawce H, Olson S, Druker B, Mauro M, Vandyke J. Clonal chromosomal abnormalities in CD34+/CD38- hematopoietic cells from cytogenetically normal chronic myeloid leukemia patients with a complete cytogenetic response to tyrosine kinase inhibitors. Leukemia (2010) 24, 1525–1528.
Pommier S, Christante D, Muller P, Newell A, Olson S, Diggs B, Muldoon L, Neuwelt E, Pommier R. Characterizing the HER2/neu status and metastatic potential of breast cancer stem/progenitor cells. Annals of Surgical Onc. 17(2):613-623, 2010.
Van de Vrugt H, Eaton L, Hanlon Newell A, Al-Dhalimy M, Liskay RM, Olson SB, Grompe M. Embryonic lethality after combined inactivation of Fancd2 and Mlh1 in mice. Cancer Research. 69: 9431-9438, 2009.
Duncan AW, Hickey RD, Paulk NK, Culberson AJ, Olson SB, Finegold MJ, Grompe M. Ploidy reductions in murine fusion-derived hepatocytes. PLoS Genet. 2009 Feb;5(2):e1000385.
Hemphill AW, Akkari Y, Hanlon Newell A, Schultz RA, Grompe M, North PS, Hickson ID, Jakobs PM, Rennie S, Pauw D, Hejna J, Olson SB, Moses RE. Topo IIIα and BLM act within the Fanconi anemia pathway in response to DNA-crosslinking agents. Cytogenet. Genome Res. 2009;125(3):165-75.
Moghrabi NN, Johnson MA, Zhu X, Al-Dhalimy M, Olson S, Grompe M, Richards CS. Validation of Fanconi Anemia Group A Subtyping Using an Integrated Strategy for Rapid and Comprehensive FANCA Molecular Analysis and Identification of Novel FANCA Mutations. Genetics in Medicine 11(3) March 2009.
Hanlon Newell AE, Hemphill A, Akkari YMN, Hejna J, Moses R, Olson SB. Loss of homologous recombination or non-homologous end-joining leads to radial formation following DNA interstrand crosslink damage. Cytogenetics and Genome Research 121(3-4):174-80, 2008.
Hejna J, Holtorf M, Hines J, Mathewson L, Hemphill A, Al-Dhalimy M, Olson SB, Moses RE: Tip60 is required for DNA interstrand crosslink repair in the Fanconi anemia pathway. J Biol Chem, 283(15):9844-51, 2008.
Hemphill AW, Bruun D, Thrun L, Akkari Y, Torimaru Y, Hejna K, Jakobs PM, Hejna J, Olson SB, Moses RE: Mammalian SNM1 is required for genome stability. Mol Genet Metab., 94(1):38-45, 2008.
Held P, Al-Dhalimy M, Willenbring H, Akkari Y, Jiang S, Torimaru Y, Olson S, Fleming W, Finegold M, Grompe M. In Vivo Genetic Selection of Renal Proximal Tubules. Molecular Therapy 13:49-58, 2006.
Maslen CL, Babcock D, Redig JK, Kapeli K, Akkari YM, Olson SB: CRELD2:Gene mapping, alternate splicing, and comparative genomic identification of the promoter region. Gene, 382:11-120, 2006.
Houghtaling S, Newell A, Akkari Y, Taniguchi T, Olson S, Grompe M. Fancd2 functions in a double strand break repair pathway that is distinct from non-homologous end joining. Hum Mol Genet. 14(20):3027-33, 2005.
Houghtaling S, Granville L, Akkari Y, Torimaru Y, Olson S, Finegold M, Grompe M. Heterozygosity for p53 (Trp53+/-) Accelerates Epithelial Tumor Formation in Fanconi Anemia Complementation Group D2 (Fancd2) Knockout Mice. Cancer Research 65:85-91, 2005.
Hanlon Newell AE, Akkari, YMN, Torimaru Y, Rosenthal A, Reifsteck C, Cox B, Grompe M, Olson SB. Interstrand crosslink-induced radials form between non-homologous chromosomes, but are absent in sex chromosomes. DNA Repair 3:535-542, 2004.
Willenbring H, Bailey AS, Foster M, Akkari Y, Dorrell C, Olson S, Finegold M, Fleming WH, Gompe M. Myelomonocytic cells are sufficient for therapeutic cell fusion in liver. Nature Medicine 10:744-748, 2004.
Wang X, Willenbring H, Akkari Y, Torimaru Y, Foster M, Al-Dhalimy M, Lagasse E, Finegold M, Olson S, Grompe M. Cell fusion is the principal source of bone-marrow-derived hepatocytes. Nature 422:897-901, 24 April 2003.