OHSU

James Whittaker

James Whittaker

Email: 
Phone:  503 748-1065
Fax:  503 748-1464
Lab Phone:  503 748-1119

 

Current Appointments

Associate Professor, Division of Environmental and Biomolecular Systems

 

Education

Ph.D. Biochemistry, University of Minnesota, 1983
B.Sc. (Hon.), Biological Sciences, Sir George Williams University, Montreal, 1978

 

Biography

  • NIH Postdoctoral Fellow, Chemistry, Stanford University, 1983-1986
  • Carnegie Mellon University, Assistant Professor, Chemistry 1983-1986, Associate Professor, Chemistry, 1992-1996

 

Research Interests

Electronic structures and dynamics of metalloenzyme active sites; spectroscopic and computational approaches to biomolecular structure; metalloenzyme mechanisms; enzyme engineering; biology of metal ions.

 

Selected Publications

Whittaker, M. M.; Whittaker, J. W. (2012) Metallation state of human manganese superoxide dismutase expressed in Saccharomyces cerevisiae. Arch. Biochem. Biophys., 523, 191-197. (DOI: 10.1016/j.abb.2012.04.016)

Whittaker, J. W. (2012) Non-heme manganese catalase - The other' catalase. Arch. Biochem. Biophys., 525, 111-120. (DOI: 10.1016/j.abb.2011.12.008)

McConnell, I. L.; Grigoryants, V. M.; Scholes, C. P.; Myers, W. K.; Chen, P.-Y.; Whittaker, J. W.; Brudvig, G. W. (2012) EPR-ENDOR characterization of (17O, 1H, 2H) water in manganese catalase and its relevance to the oxygen-evolving complex of photosystem II. J. Am. Chem. Soc., 134, 1504-1512. (DOI: 10.1021/ja203465y)

Whittaker, M. M.; Lerch, T. F.; Kirillova, O.; Chapman, M. S.; Whittaker, J. W. (2011) Subunit dissociation and metal binding by Escherichia coli apo-manganese superoxide dismutase. Arch. Biochem. Biophys., 505, 213-225. (DOI: 10.1016/j.abb.2010.10.021)

Whittaker, J. W. (2010) Metal uptake by manganese superoxide dismutase. Biochim. Biophys. Acta, Proteins Proteomics, 1804, 298-307. (DOI: 10.1016/j.bbapap.2009.08.014)

Stich, T. A.; Whittaker, J. W.; Britt, R. D. (2010) Multifrequency EPR studies of manganese catalases provide a complete description of proteinaceous nitrogen coordination. J. Phys. Chem. B, 114, 14178-14188. (DOI: 10.1021/jp908064y)

Kempner, E. S.; Whittaker, J. W.; Miller, J. H. (2010) Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical. Protein Sci., 19, 236-241. (DOI: 10.1002/pro.302)

Whittaker, M. M.; Whittaker, J. W. (2009) In vitro metal uptake by recombinant human manganese superoxide dismutase. Arch. Biochem. Biophys., 491, 69-74. (DOI: 10.1016/j.abb.2009.09.003)

Li, Z.; Moy, A.; Sohal, K.; Dam, C.; Kuo, P.; Whittaker, J.; Whittaker, M.; Duezguenes, N.; Konopka, K.; Franz, A. H.; Lin-Cereghino, J.; Lin-Cereghino, G. P. (2009) Expression and characterization of recombinant human secretory leukocyte protease inhibitor (SLPI) protein from Pichia pastoris. Protein Expression Purif., 67, 175-181. (DOI: 10.1016/j.pep.2009.06.001)

Whittaker, M. M.; Whittaker, J. W. (2008) Conformationally gated metal uptake by apomanganese superoxide dismutase. Biochemistry, 47, 11625-11636. (DOI: 10.1021/bi8015636)

Lee, Y.-K.; Whittaker, M. M.; Whittaker, J. W. (2008) The electronic structure of the Cys-Tyr* free radical in galactose oxidase determined by EPR spectroscopy. Biochemistry, 47, 6637-6649. (DOI: 10.1021/bi800305d)