Titan Krios TEM

Titan Krios™ Cryo-electron microscope

Features:

• Robotic sample handling
• Loading of 12 samples
• Parallel illumination
• Optimized for Structural (cellular) Biology applications: Cryo Tomography, single particle analysis, electron crystallography
• Dual axis tilt holder (+/- 70 degrees) enabling dual axis tomography
• Demonstrated 2.8 angstrom resolution on a virus specimen
• Information Limit: < .14 nm at zero tilt;
• STEM capabilities

Titan Krios™ Technical Bulletin

About the Krios

Titan Krios™ is a state-of-the-art, dedicated imaging robot, optimized for both high-resolution 3D imaging of proteins and macromolecular assemblies, and high-resolution, dual-axis, cellular tomography. Its revolutionary cryo-based technology and stability permits a full range of semi-automated applications, including: 2D electron crystallography, single particle analysis, cryo-electron microscopy, and dual-axis cellular tomography of frozen hydrated samples. Cryo-techniques preserve sample integrity by maintaining the sample in its natural condition and state. 

Location: Floor Zero, Richard Jones Hall
Online:  September 2012
One of 50 or fewer high-resolution, cryo-electron microscopes in the world.
Requires a specialized environment: low vibration and heat differential tolerance, an absence of electromagnetic currents.
Achieves atomic resolution data.
Good for achieving high-resolution, high-quality optics for cryo samples, as opposed to fixed samples. 
Has low-dose capabilities, achieves balance between showing good information and destroying the sample.