OHSU FEI Living LabThe OHSU FEI Living Lab is a collaborative research program in the OHSU Center for Spatial Systems Biomedicine (OCSSB) that provides access to a state-of-the-art microscopy suite and develops workflow solutions for integrated light and electron microscopy (EM) applications. The OHSU FEI Living Lab, a collaboration between OHSU and Hillsboro-based FEI, Inc., provides access to technical support, training and instrumentation, including a Helios NanoLab™ 650 Dualbeam™ (used for automated high resolution 2D and 3D SEM image acquisition and sample preparation), a Titan Krios™ Cryo-Electron microscope (which is an automated TEM platform optimized for Single Particle Analysis and 3D cryo-TEM applications - with a world record resolution of 2.8Å, and a Tecnai™ with iCorr™ (the world’s first commercial microscope which integrates light and EM capabilities).
The OHSU FEI Living Lab allows scientists to visualize cell structure and assemblies of biological macromolecules at a level of detail that wasn't possible before, enabling them to explore, among other things, how cancer cells function differently as they spread from the site of origin to other parts of the body, and how genetic mutations work together to drive cancer and other diseases. Scientists have access to early versions of next-generation microscopy systems so that they can contribute to the product, reagent, and workflow development process. The collaboration will deepen understanding of EM workflow as applied to cellular biology and, ultimately guide the development of next-generation tools.
ELECTRON MICROSCOPY SUITE
All academic and commercial users are welcome to utilize the OHSU FEI living lab microscopes. Technical support and training are provided by the OHSU EM Core and FEI collaborators. We offer multiple levels of service that correspond with the analysis and/or training each user desires. Training on the instruments will be provided for individuals who anticipate having long-term EM projects.
• FEI Titan Krios™ Cryo-electron microscope (TEM)
• FEI Helios™ Nanolab™ 650 Dual Beam scanning electron microscope (SEM)
• FEI Tecnai integrated light-electron microscope (iLEM)
cryo-Ultramicrotome (Leica EM FC7)
High-pressure freezer (Wohlwend Compact 01)
Freeze Substitution & Low Temperature Embedding System (Leica EM AFS2)
Pelco easiGlow 91000 Glow Discharge Cleaning System