Tecnai Correlative EM

About the Correlative Electron Microscope

 The most critical "tie point" between imaging modalities is between fluorescence (FM) and electron microscopy (EM), with EM providing Angstrom to nanometer resolution information about molecular ultrastructure and FM providing nanometer to micrometer resolution information about functional molecular complexes.  Scientists from FEI and the Biomolecular Imaging program at Utrecht University have developed novel sample fixation and staining procedures for the Tecnai™ Correlative EM that preserve both ultrastructural and molecular features for FM and transmission electron microscopy (TEM) and reduce sample preparation times.

Location: Multnomah Pavilion
Online: November 2012
One of two in the world (the other is located in the Netherlands); this one is the first to be commercially available.
Correlates light microscopy and electron microscopy. Uses light microscopy to find a rare cell or event and uses electron microscopy to study that event.
Ideal for identifying rare cells, events in a large sample, such as 1 in 10,000 events or cells.

Published Images

 From Traffic, Volume 12, Issue 7, pages 806-814, 28 APR 2011
iCorr imaging of VIS2FIXFSsections labeled for LAMP-2. A) Fluorescence image of a 50 µm2 area on a section labeled for LAMP-2 with immuno-gold (15 nm) and immuno-fluorescence (Alexa 488 Fluor, see Online Methods). B) Overlay of the fluorescence signal of the area indicated in A (white square), and a TEM image of the same region. C) A TEM image of the area indicated in B (black square). Here it becomes clear that the lysosomes are abundantly labeled. Scale bars represent 10 µm in A, 1 µm in B, and 500 nm in C.

Reproduced with permission