Focused ion beam scanning electron microscopy (FIB-SEM)
Focused ion beam scanning electron microscopy (FIB-SEM), or "slice and view," allows 3D imaging of en bloc biological samples with nanometer resolution. During image acquisition, the FIB mills thin sections of about 4 nm thick from the block, and then the SEM images the block face. This is done repeatedly to obtain a 3D image of the sample. In our work we use the FEI Helios 650 NanoLab™ DualBeam™ system from the OHSU Multiscale Microscopy Core. We image cardiac tissues from chicken embryos during early heart development.
Slice-and-view image of the HH24 chick embryo heart outflow tract endocardium. The endocardium is a monolayer of endocardial cells that is in contact with intracardiac blood flow.
Slice-and-view image of the HH24 chick embryo heart outflow tract cardiac jelly layer. The cardiac jelly is predominantly an extracellular matrix layer in between the contractile myocardium layer and the endocardium layer.
Slice-and-view image of a portion of the HH24 chick embryo heart outflow tract myocardium. The myocardium is a compact layer of myocardial cells that actively contracts to pump blood through the circulation. The myocardium is also in contact with the cardiac jelly, and portions of the cardiac jelly can be observed in this movie.