Gibbs Laboratory Research Overview
The Gibbs Laboratory is focused on the development of novel imaging reagents for improved macroscopic and microscopic cancer imaging applications.
Fluorophores for Macroscopic Imaging
In the macroscopic imaging arena our efforts are concentrated on developing tissue- and disease-specific fluorophores to guide oncologic surgery. Currently we are focused on the development of near infrared nerve-specific fluorophores that can be utilized during oncologic surgeries where preservation of intact nerve tissue is paramount for quality of life post surgical intervention such as nerve-sparing radical prostatectomy. We are also heavily invested in the development of targeted fluorescent agents for intraoperative tumor margin assessment, where our current emphasis is on the development of small molecule fluorophore conjugates to highlight tumor margins during Whipple procedures for pancreatic cancer.
High and Superresolution Microscopy
On the other end of the size spectrum, we are developing novel fluorescent reagents for improved high-resolution microscopy with specific focus on super resolution microscopy and correlative light and electron microscopy (CLEM). We are working to collaboratively develop a spectral extension of superresolution microscopy that will enable up to 20-color simultaneous imaging in a single sample, which is being applied to improve our understanding of breast cancer and breast cancer treatment. We are also focused on the development of caged fluorophores for two-photon photoactivation to enable three-dimensional superresolution microscopy in whole cells and relatively thick tissue specimens. To enable improved contrast for CLEM, we are currently characterizing various fluorophore scaffolds for their utility in the presence of electron microscopy sample preparation reagents with future plans to nanoparticle encapsulate the most robust fluorophore scaffolds to improve CLEM imaging.