Interactions between Neurons, Astrocytes and the Vascular
Anusha Mishra Laboratory, OHSU Knight Cancer Institute
The Mishra lab aims to unravel the mechanisms underlying the disruption of neurovascular coupling in stroke. A post-doc position is open to carry out experiments along these lines. One primary project is investigating the mechanisms by which reactive astrocytes might precipitate a mismatch between neuronal activity and cerebral blood flow. A second project is geared towards developing a novel AAV construct that allows stable expression of genetically encoded calcium indicators in astrocytes without being influenced by the status of astrocyte reactivity, thus enhancing our ability to study astrocyte signaling in disease processes.
The post-doc will be responsible for experimental design, data collection and interpretation, and preparation of the data for publication in peer reviewed journals. Specific responsibilities include but are not limited to:
- conducting animal surgery (transient focal cerebral ischemia models)
- AAV-mediated gene transfer
- brain slice preparation
- fluorescence, IR-DIC and confocal microscopy
- calcium imaging
- in vivo imaging
- behavioral experiments
- caretaking of the animals
The post-doc will also facilitate lab organization, participate in regular lab meetings, attend research seminars and journal clubs, present their work at national/international conferences and assist in training new people in the lab. Experience in any of the above techniques and a willingness to learn are the main criteria. Ideally, the post-doc would be able to start in the lab by late summer/early fall of 2017. Please email curriculum vitae and contact information of 2-3 references to firstname.lastname@example.org to arrange an interview.