OHSU

Graduate Studies Faculty

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Eric Barklis, PhD

Professor
Director, OHSU EM Core Facility
Admin Unit: SOM-Molecular Microbiology & Immunology Department
Lab Phone: 503-494-8098
Fax: 503-494-6862
Office: RJH 6369
Mail Code: L220
Programs:
Molecular Microbiology & Immunology
Neuroscience Graduate Program
Program in Molecular & Cellular Biosciences
Cancer Biology
Research Interests:
biochemistry structural biology biophysics molecular biophysics nucleic acids virology electron microscopy protein structure membrane biology HIV chemical biology drug development microbial pathogenesis virus assembly virus structure » Click here for more about Dr. Barklis's research
Preceptor Rotations
Dr. Barklis has not indicated availability for preceptor rotations at this time.
Faculty Mentorship
Dr. Barklis has not indicated availability as a mentor at this time.
Profile

Background

Eric Barklis received a B.A. degree in Biology from the Massachusetts Institute of Technology in 1973. He began graduate study in English Literature at Northeastern University, then returned to MIT for a Ph.D. in Biochemistry. After three years of postdoctoral study at the Whitehead Institute, Barklis received joint faculty appointments in the Vollum Institute and the Department of Microbiology in 1987. He was promoted to associate professor in 1994, became director of the OHSU Electron Microscopy core in 1999, and was promoted to professor in 2001.

Summary of Current Research

Research in the Barklis lab focuses on the assembly and replication of viruses, such as retroviruses, flaviviruses, and hantaviruses, using molecular genetic, biochemical, and biophysical techniques. Molecular genetic and biochemical approaches are employed to investigate viral protein interactions, RNA recognition and encapsidation, and cellular factors involved in virus replication and assembly. To analyze virus particles, proteins, and macromolecular complexes, a variety of biophysical methods are utilized, including sedimentation, crosslinking, fluorescence microscopy, fluorescence anisotropy, transmission electron microscopy (EM), and atomic force microscopy (AFM). One set of recent investigations concentrates on the identification and analysis of small molecule inhibitors of virus replication. A second avenue of inquiry concerns the mechanisms that govern how HIV structural proteins assemble conical, cylindrical and spherical cores. Our third major area of research focuses on protein-protein and protein-lipid interactions of retrovirus membrane-binding proteins. Ultimately, we believe our studies will lead to the development of new antivirals, and a better understanding of the basic principles controlling macromolecular assembly.

Selected Publications

Scholz, I., Still, A., Dhenub, T.C., Coday, K., Webb, M., and Barklis, E. (2008) Analysis of human immunodeficiency virus matrix domain replacements. Virology 371:322-335.

Alfadhli, A., Huseby, D., Kapit, E., Colman, D., and Barklis, E. (2007) The HIV-1 matrix protein assembles on membranes as a hexamer. J. Virol. 81:1472-1478.

Barklis, E., Still, A., Sabri, M.I., Hirsch, A.J., Nikolich-Zugich, J., Brien, J., Dhenub, T.C., Scholz, I., and Alfadhli, A. (2007) Sultam thiourea inhibition of West Nile virus. Antimicrob. Agents Chemother. 51:2642-2645.

Alfadhli, A., Dhenub, T.C., Still, A., and Barklis, E. (2005) Analysis of human immunodeficiency virus type 1 Gag dimerization-induced assembly. J. Virol. 79:14498-14506.

Huseby, D., Barklis, R.L., Alfadhli, A., and Barklis, E. (2005) Assembly of human immunodeficiency virus precursor Gag proteins. J. Biol. Chem. 280:17664-17670.

Scholz, I., Arvidson, B., Huseby, D., and Barklis, E. (2005) Virus particle core defects caused by mutations in the human immunodeficiency virus capsid N-terminal domain. J. Virol. 79:1470-1479.