Advances in Research

Key and Recent Publications:

Michael Axthelm, DVM

Axthelm MK, Koralnik IJ, Dang X, Wuthrich C, Rohne D, Stillman IE, Letvin NL. Meningoencephalitis and demyelination are pathologic manifestations of primary polyomavirus infection in immunosuppressed rhesus monkeys (2004). J Neuropathol Exp Neurol 63(7):750-758.

Picker LJ, Hagen SI, Lum R, Reed-Inderbitzin EF, Daly LM, Sylwester AW, Walker JM, Siess DC, Piatak Jr M, Wang C, Allison DB, Maino VC, Lifson JD, Kodama T, Axthelm, MK (2004). Insufficient production and tissue delivery of CD4+memory T cells in rapidly progressive simian immunodeficiency virus infection. J Exp Med 200(10):1299-1314. PMCID: PMC2211921.

Okoye A, Meier-Schellersheim M, Brenchley JM, Hagen SI, Walker JM, Rohankhedkar M, Lum R, Edgar JB, Planer SL, Legasse A, Sylwester AW, Piatak M Jr, Lifson JD, Maino VC, Sodora DL, Douek DC, Axthelm MK, Grossman Z, Picker LJ (2007). Progressive CD4+ central memory T cell decline results in CD4+ effector memory insufficiency and overt disease in chronic SIV infection. J Exp Med 204(9):2171-2185.  PMCID: PMC2118701.

Patrizia Caposio, PhD

Caposio P, Dreano M, Garotta G, Gribaudo G and Landolfo S (2004). Human cytomegalovirus stimulates cellular IKK2 activity and requires the enzyme for productive replication. JVirol 78: 3190-3195.  PMCID: PMC353729

Caposio P, Musso T, Luganini A, Inoue H, Gariglio M, Landolfo S and Gribaudo G (2007). Targeting the NF-kappaB pathway through pharmacological inhibition of IKK2 prevents human cytomegalovirus replication and virus-induced inflammatory response in infected endothelial cells. Antiviral Res 73(3):175-84.

Caposio P, Luganini A, Hahn G, Landolfo S and Gribaudo G (2007). Activation of the virus-induced IKK/NF-kappaB signalling axis is critical for the replication of human cytomegalovirus in quiescent cells. Cell Microbiol 9(8):2040-54.

Luganini A, Caposio P, Landolfo S and Gribaudo G (2008). Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry. Antimicrob Agents Chemother 52(3):1111-20.  PMCID: PMC2258505

Luganini A, Caposio P, Landolfo S and Gribaudo G (2008). New cell-based indicator assays for the detection of human cytomegalovirus infection and screening of inhibitors of viral immediate-early 2 protein activity. J Appl Microbiol 105(6):1791-801.

Victor Defillipis, PhD

DeFilippis VR, Alvarado D, Sali T, Rothenburg S, and Früh K. Human cytomegalovirus induces the interferon response via the DNA receptor ZBP1 (under review).

DeFilippis VR, Robinson B, Keck T, Hansen SG, Nelson JA and Früh K (2006). Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection. J Virol 80:1032-1037.  PMCID: PMC1346858.

DeFilippis VR and Früh K (2005). Inhibition of interferon regulatory factor 3 activation by rhesus cytomegalovirus virions. J Virol 79:6419–6431.

DeFilippis VR and Villarreal LP (2001). Virus evolution. Chapter 13 in Fields Virology, edited by D.M. Knipe and P.M. Howley. Lippincott - Raven Publishers. Philadelphia.

Klaus Früh, PhD
Alzhanova D, Edwards DM, Hammarlund E, Scholz IG, Horst D, Wagner MJ, Upton C, Wiertz EJ, Slifka MK  and Früh . (2009). Cowpox virus inhibits Transporter associated with Antigen Processing to evade T cell recognition. Cell Host & Microbe in press.

Mansouri M, Viswanathan K, Douglas JL, Hines J, Gustin J, Moses AV and Früh K (2009). Molecular mechanism of BST2/Tetherin downregulation by K5/MIR2 of Kaposi's sarcoma herpesvirus. J Virol, in press.

Hammarlund E, Dasgupta A, Pinilla C, Norori P, Früh K and Slifka MK (2008). Monkeypox virus evades antiviral CD4+ and CD8+ T cell responses by suppressing cognate T cell activation. Proc Natl Acad Sci U S A 105, 14567-14572.  PMCID: PMC2567221.

Mansouri M, Rose PP, Moses AV and Früh K (2008). Remodeling of endothelial adherens junctions by Kaposi's sarcoma-associated herpesvirus. J Virol 82, 9615-9628.  PMCID: PMC2546942.

Powers CJ and Früh K (2008). Signal peptide-dependent inhibition of MHC class I heavy chain translation by rhesus cytomegalovirus. PLoS Pathog 4, e1000150.  PMCID: PMC2542416.

Bartee E, McCormack A and Früh K. (2006). Quantitative membrane proteomics reveals new cellular targets of viral immune modulators. PLoS Pathog 2, e107.  PMCID: PMC1626102.

Nancy Haigwood, PhD

Steimer KS, Scandella, CJ, Skiles, PV, and Haigwood, NL (1991). Neutralization of divergent HIV-1 isolates by conformation-dependent human antibodies to gp120. Science 254:105-08.

Haigwood NL, DC Montefiori, WF Sutton, J McClure, A Watson, G Voss, VM Hirsch, B Richardson, NL Letvin, S-L Hu and PR Johnson (2004). Passive Immunotherapy in SIV-Infected Macaques Accelerates the Development of Neutralizing Antibodies. J Virol 78:5983-95.  PMCID: PMC415787.

Jayaraman P, T Zhu, L Misher, D Mohan, L Kuller, P Polacino, BA Richardson, H Bielefelt-Ohmann, D Anderson, S-L Hu, and NL Haigwood (2007) Evidence for Persistent, Occult Infection in Neonatal Macaques following Perinatal Transmission of SHIV-SF162P3, J Virol 81: 822-834.  PMCID: PMC1797486.

Blay WM, T Kasprzyk, L Misher, BA Richardson, NL Haigwood (2007). Mutations in Envelope gp120 can impact proteolytic processing of the gp160 precursor and thereby affect neutralization sensitivity of human immunodeficiency virus type 1 pseudoviruses. J Virol 81: 13037-13049.  PMCID: PMC2169095

Morgan C, Marthas M, Miller C, Duerr A, Cheng-Mayer C, Desrosiers R, Flores J, Haigwood N, Hu SL, Johnson RP, Lifson J, Montefiori D, Moore J, Robert-Guroff M, Robinson H, Self S, Corey L. (2008) The use of nonhuman primate models in HIV vaccine development. PLoS Med 5(8):e173.   PMCID: PMC2504486.

M Mahalanabis, P Jayaraman, T Miura, F Pereyra, EM Chester, B Richardson, B Walker, and NL Haigwood (2009). Continuous Viral Escape and Selection by Autologous Neutralizing Antibodies in Drug-Naïve HIV Controllers, J Virol 83: 662-672.  PMCID: PMC2612349.

Michael Jarvis, PhD

Dutia BM, Reid SJ, Drummond DD, Ligertwood Y, Bennet I, Rietberg W, Silvia O, Jarvis MA, Nash AA (2009). A novel Cre recombinase imaging system for tracking lymphotropic virus infection in vivo. PLos One 4:e6492.  PMCID: PMC2714982.

Hansen SG, Vieville C, Whizin N, Coyne-Johnson L, Siess DC, Drummond DD, Legasse AW, Axthelm MK, Oswald K, Trubey CM, Piatak M Jr, Lifson JD, Nelson JA, Jarvis MA, Picker LJ (2009).  Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge.  Nat Med 15:293.  PMCID: PMC2720091.

Jarvis MA, Nelson JA (2007). Human cytomegalovirus tropism for endothelial cells: not all endothelial cells are created equal. J Virol 81:2095.  PMCID: PMC1865914.

Jarvis MA, Borton JA, Keech AM, Wong J, Britt WJ, Magun BE, Nelson JA (2006).  Human cytomegalovirus attenuates interleukin-1 beta and tumor necrosis factor alpha proinflammatory signaling by inhibition of NF-kappaB activation. J Virol 80:5588.  PMCID: PMC1472148.

Ilhem Messaoudi, PhD
Messaoudi I, Warner J, Nikolich-Zugich D, Fischer M, and Nikolich-Zugich J (2006). Molecular, cellular and antigen requirements for development of age-associated T cell clonal expansions in vivo. J Immunol 176(1): 301-8. 

Messaoudi I, Warner J, Fischer M, Park B, Hill B, Mattison J, Lane MA, Roth G S, Ingram D K, Picker LJ, Douek DC, Mori M, and Nikolich-Zugich J (2006). Delay of T cell senescence by caloric restriction in aged long-lived non-human primates. Proc Natl Acad Sci U S . 103(51): 19448-53.  PMCID: PMC1748246.

Amana I, Messaoudi I, and Slifka MK (2008). Protective Immunity following vaccination: How is it defined? Human Vaccines (4): 316-9.

Messaoudi I, Blossom D, and Wong SW (2008). Primate Models for Gammaherpesvirus-associated Malignancies. DNA Tumor Viruses, chapter 27, Springer.

Messaoudi I, Fischer MB, Warner J, Park B, Mattison J, Ingram DK, Totonchy T, Mori M, and Nikolich-Zugich J (2008). Optimal window of caloric restriction onset limits its beneficial impact upon T cell senescence in primates. Aging Cell (6): 908-19.  PMCID: PMC2659568.

Ashlee Moses, PhD

Douglas JL, Whitford JG and Moses AV (2009). Characterization of c-Kit expression and activation in KSHV-infected endothelial cells. Virology 390:174-85.
Douglas JL, Viswanathan K, McCarroll MN, Gustin JK,  Früh K and Moses AV (2009). Vpu directs the degradation of the HIV restriction factor BST-2/tetherin via a bTrCP-dependent mechanism. J Virol 83:7931-47.  PMCID: PMC2715753 [Available on 2010/02/01].

Jay Nelson, PhD

Dumortier J, Streblow DN, Moses AV, Jacobs JM, Kreklywich CN, Camp D, Smith RD, Orloff SL and Nelson JA (2008). Human cytomegalovirus secretome contains factors that induce angiogenesis and wound healing. J Virol 82:6524-35.  PMCID: PMC2447085.
Nelson JA (2007). Small RNAs and Large DNA viruses. N Engl J Med 357: 25-27.

Grey F, Meyers H, White EA, Spector DH and Nelson JA (2007). A human cytomegalovirus-encoded microRNA regulates expression of multiple viral genes involved in replication. PLOS Pathogens 3 (11):e163.  PMCID: PMC2048532.

Hirsch A, Medigeshi G, Meyers H, De Fillippis V, Fruh K, Briese T, Lipkin WI and Nelson JA (2005). The Src Family Kinase c-Yes is Required for Maturation of West Nile Virus Particles. J Virol 79(18):11943-11951.  PMCID: PMC1212639.

Varnum SM, Streblow DN, Monroe ME, Smith P, Aubrey KJ, Pa_a-Toli L, Wang D, Camp DG II, Rodland K, Wiley S, Britt W, Shenk T, Smith RD and Nelson JA (2004). Identification of proteins in human cytomegalovirus (HCMV) particles: The HCMV proteome. J Virol 78:10960-6.  PMCID: PMC521840.

Streblow DN, Vomaske J, Smith P, Melnychuk R, Hall L, Pancheva D, Schlaephler DA, and Nelson JA (2003). The human cytomegalovirus chemokine receptor US28 activates focal adhesion kinase in a ligand dependent manner. J Biol Chem 278(50):50456-65.
Haesun Park, Phd
Park HS and Cleary PP (2005). Active and passive immunizations with the streptococcal surface protein C5a peptidase prevent infection of murine nasal-associated lymphoid tissue, a functional homologue of human tonsils. Infect. Immun 73:7878-7886.  PMCID: PMC1307028.

Park HS, Costalonga M, Reinhardt RE, Dombek PE, Jenkins MK and Cleary PP (2004). Primary induction of CD4 T cell responses in nasal associated lymphoid tissue during Group A streptococcal infection. Eur. J Immunol 34: 2843-2853.

Park HS, Francis KP, Yu J and Cleary PP (2003). Membranous cells in nasal-associated lymphoid tissue: a portal of entry for the respiratory mucosal pathogen Group A Streptococcus. J Immunol 171: 2532-2537.

Louis Picker, MD

Grossman Z, Meier-Schellersheim M, Paul WE, and Picker LJ (2006). Pathogenesis of HIV infection: What the virus spares is as important as what it destroys Nat Med12:289-295.

Picker LJ, Reed-Inderbitzin EF, Hagen SI, Edgar JB, Hansen, SG, Legasse, A, Planer S, Piatak, Jr., M, Lifson, JD, Maino VC, Axthelm MK, and Villinger F (2006). IL-15 induces CD4+ effector memory T cell production and tissue emigration in non-human primates. J Clin Invest116:1514-1524.  PMCID: PMC1459071.

Okoye A, Meier-Schellersheim M, Brenchley JM, Hagen SI, Walker JM, Rohankhedkar M, Lum R, Edgar J, Planer SL, Legasse A, Sylwester AW, Piatak, Jr., M, Lifson, JD, Maino VC, Sodora DL, Douek DC, Axthelm MK, Grossman Z, and Picker LJ (2007). Progressive CD4+ central memory T cell decline results in CD4+ effector memory insufficiency and overt disease in chronic SIV infection. J Exp Med 204:2171-2185.  PMCID: PMC2118701.

Price DA, Bitmansour AD, Walker JM, Edgar JB, Axthelm MK, Douek DC, and Picker LJ (2008). Induction and evolution of cytomegalovirus-specific CD4+ T cell clonotypes in rhesus macaques. J Immunol 180:269-280. 
Hansen SG, Vieville C, Whizin N, Coyne-Johnson L, Siess DC, Drummond DD, Legasse AW, Axthelm  MK, Oswald K, Trubey CM, Piatak, Jr. M, Lifson JD, Nelson JA, Jarvis  MA, and Picker LJ (2009).  Effector-memory T cell responses are associated with protection of rhesus monkeys from mucosal SIV challenge. Nat Med 15(3):293-299.  PMCID: PMC2720091 [Available on 2009/09/01].

Mark Slifka, PhD

Hammarlund, E., M.W. Lewis, S.G. Hansen, L.I. Strelow, J.A. Nelson, G.J. Sexton, J.M. Hanifin, M.K. Slifka (2003). Duration of antiviral immunity following smallpox vaccination. Nat. Med. 9(9):1131-1137.

Hammarlund, E., Lewis, M.W., Carter, S., Yoshihara, P., Hanifin, J., Hansen, S.G., Wong, S., Strelow, L.I., Amanna, I., and M.K. Slifka (2005). Multiple diagnostic techniques identify previously immunized individuals with protective immunity against monkeypox. Nat. Med. 11(9):1005-1011.

Beadling, C., and M.K. Slifka (2006).  Quantitation of viable virus-specific T cells withouth a prior knowledge of fine epitope specificity.  Nat. Med. 12(10:1208-1212. 

Amanna, I.J., Carlson, N.E., and M.K. Slifka (2007). Duration of humoral immunity to common virus and vaccine antigens. New England Journal of Medicine 357;19:1903-1915.

Hammarlund, E., Dasgupta, A.K., Pinilla, C., Norari, P., Früh, K., and M.K. Slifka (2008).  Monkeypox virus evades antiviral CD4+ and CD8+ T cell responses by blocking cognate T cell activation.  PNAS Sep 23; 105(38): 14567-14572.  PMCID: PMC2567221
Amanna, I.J., and M.K. Slifka (2009).  Wanted, Dead or Alive:  New Viral Vaccines.  Antiviral Research: in press.

Daniel Streblow, PhD

Streblow DN, Soderberg-Naucler C, Vieira J, Smith P, Ruchti F, Mattison K and Nelson JA (1999). Vascular smooth muscle cell migration is induced by the Human Cytomegalovirus chemokine receptor US28. Cell 99, 511-21.

Streblow DN, van Cleef KW, Kreklywich CN, Meyer C, Smith P, Defilippis V, Grey F, Fruh K, Searles R, Bruggeman C, Vink C, Nelson JA and Orloff SL (2007). Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. J Virol 81(8):3816-26.  PMCID: PMC1866122.

Streblow DN, Kreklywich CN, Andoh T, Moses AV, Dumortier J, Smith PP, Defilippis V, Fruh K, Nelson JA, and Orloff SL (2008). The Role of Angiogenic and Wound Repair Factors During CMV-Accelerated Transplant Vascular Sclerosis in Rat Cardiac Transplants. Amer J Transpl Feb;8(2):277-87.

Baca Jones CC, Kreklywich CN, Messaoudi I, Vomaske J, McCartney E, Orloff SL, Nelson JA and DN Streblow (2009). Rat cytomegalovirus infection depletes MHC I and II in bone marrow derived dendritic dells. Virol 25;388(1):78-90. Epub 2009 Apr 5.

Vomaske J, Melnychuk RM, Smith PP, Powell J, Hall L, DeFillipis V, Früh K, Smit M, Schlaepfer DD, Nelson JA and DN Streblow (2009). Differential ligand binding to a human cytomegalovirus chemokine receptor determines cell type-specific motility. PLOS Pathogens 5(2) e10000304. Epub 2009 Feb 20.  PMCID: PMC2637432.

Scott Wong, PhD
Okroj M, Mark L, Stokowska A, Wong SW, Rose N, Blackbourn DJ, Villoutreix BO, Spiller OB, Blom AM (2009). Characterization of the complement inhibitory function of rhesus rhadinovirus complement control protein (RCP). J Biol Chem 284:505-514.

Schmitt K, Hill MS, Ruiz A, Culley N, Pinson DM, Wong SW, Stephens EB (2009). Mutations in the highly conserved SLQYLA motif of Vif in a simian-human immunodeficiency virus result in a less pathogenic virus and are associated with G-to-A mutations in the viral genome. Virol 383:362-372.

Orzechowska BU, Powers MF, Sprague J, Li H, Yen B, Searles RP, Axthelm MK, Wong SW (2008). Rhesus macaque rhadinovirus-associated Non-Hodgkin's lymphoma: animal model for KSHV-associated malignancies. Blood 112:4227-4234.  PMCID: PMC2582004 [Available on 2009/11/15].

Mottaz-Brewer HM, Norbeck AD, Adkins JN, Manes NP, Ansong C, Shi L, Rikihisa Y, Kikuchi T, Wong SW, Estep RD, Heffron F, Pasa-Tolic L, Smith RD (2008). Optimization of proteomic sample preparation procedures for comprehensive protein characterization of pathogenic systems. J Biomol Tech 19:285-25.

Manes NP, Estep RD, Mottaz HM, Moore RJ, Clauss TRW, Monroe ME, Adkins JN, Wong SW, Smith RD. 2008. Comparative proteomics of human monkeypox and vaccinia intracellular mature and extracellular enveloped virions. J Proteome Res7:960-968.  PMCID: PMC2517256.

Estep RD, Powers MF, Yen B, Li H, Wong SW (2007). Construction of an infectious rhesus rhadinovirus (RRV) bacterial artificial chromosome (BAC) for the analysis of Kaposi's sarcoma-associated herpesvirus (KSHV) related disease development. J Virol 81:2957-2969.