The VGTI Monoclonal Core offers monoclonal and polyclonal antibody production (DNA immunization) in mice and purification of antibody produced from existing cell lines (a stand-alone phase IV). A monoclonal project is typically done in four stages.
|4 mice are given 2-3 injections with protein antigen. Antisera titers are determined to identify animals for fusion.|
|Stage Two||Fusion and hybridoma screening|
|Spleen cells from 2 mice are fused with mouse myeloma cells to generate hybridomas. After ELISA screening, hybridoma supernates are delivered to the client for testing.|
|Stage Three||Cloning and characterization of hybridomas|
|Parent hybridomas are limit dilution cloned, providing for stable growth and antibody production and monoclonality.|
|Stage Four||Antibody production and purification|
|In vitro scaleup in bioreactors provides antibody in the 75-100 mg range.
Antibodies are purified to greater than 90% using two chromatography steps.
|TWO||Fusion and screening||1500|
|THREE||Cloning||300 (per clone)|
|FOUR||Scaleup Antibody production/purification||1650 (per Ab)|
|Small scale antibody production/purification|
|200 mL medium (2-7 mg yield)||$300|
|400 mL medium (4-14 mg yield)||$500|
|Prices are subject to change. Please contact Dr. Daniel Cawley for further information: