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VGTI in the News

The Art of Self- Defence (Nature) (click to download)

The VGTI Monoclonal Core offers monoclonal and polyclonal antibody production (DNA immunization) in mice and purification of antibody produced from existing cell lines (a stand-alone phase IV).  A monoclonal project is typically done in four stages.

Stage One Immunization
4 mice are given 2-3 injections with protein antigen. Antisera titers are determined to identify animals for fusion.
Stage Two Fusion and hybridoma screening
Spleen cells from 2 mice are fused with mouse myeloma cells to generate hybridomas.  After ELISA screening, hybridoma supernates are delivered to the client for testing.
Stage Three Cloning and characterization of hybridomas
Parent hybridomas are limit dilution cloned, providing for stable growth and antibody production and monoclonality.
Stage Four Antibody production and purification
In vitro scaleup in bioreactors provides antibody in the 75-100 mg range. 
Antibodies are purified to greater than 90% using two chromatography steps.
I Immunization 850 950
II Fusion and screening 1600 1900
III Cloning 350 (per clone) 375 (per clone)
IV.1 200 mL medium (2-7 mg yield) 350 (per Ab) 400 (per Ab)
IV.2 400 mL medium (4-14 mg yield) 550 (per Ab) 600 (per Ab)
IV.3 bioreactor (mininum yield 75 mg) 1750 (per Ab) 1900 (per Ab)
Prices are subject to change. Please contact Dr. Daniel Cawley for further information: