The VGTI Monoclonal Core offers monoclonal and polyclonal antibody production (DNA immunization) in mice and purification of antibody produced from existing cell lines (a stand-alone phase IV). A monoclonal project is typically done in four stages.
|4 mice are given 2-3 injections with protein antigen. Antisera titers are determined to identify animals for fusion.|
|Stage Two||Fusion and hybridoma screening|
|Spleen cells from 2 mice are fused with mouse myeloma cells to generate hybridomas. After ELISA screening, hybridoma supernates are delivered to the client for testing.|
|Stage Three||Cloning and characterization of hybridomas|
|Parent hybridomas are limit dilution cloned, providing for stable growth and antibody production and monoclonality.|
|Stage Four||Antibody production and purification|
|In vitro scaleup in bioreactors provides antibody in the 75-100 mg range.
Antibodies are purified to greater than 90% using two chromatography steps.
|PHASE||PROGRAM||OHSU Cost||External Rate|
|II||Fusion and screening||1600||1900|
|III||Cloning||350 (per clone)||375 (per clone)|
|IV.1||200 mL medium (2-7 mg yield)||350 (per Ab)||400 (per Ab)|
|IV.2||400 mL medium (4-14 mg yield)||550 (per Ab)||600 (per Ab)|
|IV.3||bioreactor (mininum yield 75 mg)||1750 (per Ab)||1900 (per Ab)|
|Prices are subject to change. Please contact Dr. Daniel Cawley for further information: