Categories | Inventors
pLXSN human KIT V654A + D816Y
OHSU # 1658-E
Categories:
Inventors:
- Michael Heinrich, SM.Medicine
Technology Overview
This line was
created by ENU-mutagenesis of BaF3 pLXSN KIT D816Y cell line and drug selection.
The V654A mutation is associated with imatinib-resistance in GIST. This cell
line expresses high levels of activated KIT. In addition to the engineered D816Y
mutation, the cells also acquired the following mutations due to retrovirus
recombination events: N587S and deletion of amino acids 931-961. These secondary
events do not appear to alter the kinase properties of KIT or alter the pattern
of D816Y sensitivity/resistance to kinase inhibitors. This cell line is strongly
resistant to imatinib.
For more information, contact:
Dennis Hanson
Technology Development Manager
503-494-5861
