Categories | Inventors
pLXSN human KIT V654A + D816F
OHSU # 1658-D
Categories:
Inventors:
- Michael Heinrich, SM.Medicine
Technology
Overview
This line was created by ENU-mutagenesis of BaF3 pLXSN KIT
D816F cell line and subsequent drug selection. The V654A mutation is associated
with imatinib-resistance in GIST. This cell line expresses high levels of
activated KIT. In addition to the engineered D816F mutation, the cells also
acquired the following mutations due to retrovirus recombination events: I571M
and M903I. These secondary events do not appear to alter the kinase properties
of KIT or alter the pattern of KIT D816F sensitivity/resistance to kinase
inhibitors. This cell line is strongly resistant to
imatinib.
For more information, contact:
Dennis Hanson
Technology Development Manager
503-494-5861
