BB19 Cell Line
OHSU # 1289
The inventors developed an immortalized human brain capillary endothelial cell line named BB19. BB19 cells are derived from human brain endothelium and are immortalized with E6E7 genes of human papilloma virus. This cell line has been used to study cytoadherence of Plasmodium falciparum-infected erythrocytes in vitro.
The cells partly maintain endothelial properties as shown by tubule formation with MatrigelTM as substrate. They express Factor VIII-related antigen and von Willebrandís factor. The cells exhibit cobblestone like morphology. The cells express P-glycoprotein (P-gp), MRP4 and BCRP, typical ABC-transporters located in the blood-brain barrier. MRP!, MRP2, MRP5, OAT3 and OAT4 have also been detected. Functional properties of the cells have been shown with the uptake of propranolol, morphine and sucrose. Uptake studies with daunomycin and the P-gp inhibitor verapamil have shown functional activity of P-gp.
This cell line can be used as a human in vitro model of the blood-brain barrier for drug uptake studies.
Dr. Jay Nelson joined the Oregon Health & Science University as a Professor in the Department of Molecular Microbiology and Immunology in 1992. He was also the Head of Pathobiology and Immunology at the Oregon National Primate Research Center from 1998 to 2000. He is currently the Director of the Vaccine and Gene Therapy Institute and holds joint appointments at the Oregon National Primate Research Center and the Department of Molecular Microbiology and Immunology.
J.G. Prudhomme et al. Int. J. Parasitol. 26 (1996) 647-655.
OHSU 1289 is available for non-exclusive licensing.
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