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Mouse CD74: Design, codon optimization, cloning, expression in E coli, and purification

 

OHSU # 2187

Technology Overview:

Protein: Recombinant mouse CD74

 

This recombinant mouse CD74 is a 191 amino acid residue polypeptide that lacks the C-terminal thyroglobulin domain. It is an E. coli expressed mouse protein that includes the intracellular domain (N-terminal amino acid residues 1 to 30), the transmembrane domain (amino acid residues 31 to 55) and the extracellular domain (amino acid residues 56 to 191). This protein would be useful for studies aimed at exploring protein-protein interactions, for blocking experiments of the MIF cytokine activity and could be useful to explore its interaction with other proteins that have been suggested to bind CD74.

 

Applications: Available as a research reagent, the protein can be used for protein-protein interactions; blocking experiments of MIF (macrophage inhibitory factor) cytokine activity; and interactions with other proteins that have been suggested to bind CD74.

 

Biological activity: This protein has been tested for its ability to bind MIF1 (or MIF), for its ability to bind several monoclonal antibodies currently used in CD74 biology research and for its capabilities to bind to recombinant human and mouse Class II proteins. Antibody-immobilized CD74 has been tested and shown to bind MIF in an in vitro assay.

 

Stability: When stored in low non-ionic detergent with 15% glycerol at -80˚C this protein is stable for at least 6 months with no appreciable degradation or reduction in its biological activity.

 

 

Inventors:

Related Technologies:

  • OHSU # 2219 — Mouse/human chimera CD74: Design, codon optimization, cloning, expression in E coli, and purification

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Michele Gunness
Senior Technology Development Manager
503-494-4184