SK3 Floxed Allele Mice (SK3 Null Mice)
OHSU # 1187
In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential. Three SK channel subunits have been molecularly characterized. These channels are found in many types of neurons as well as in some other cell types.
Previously, Dr. Adelman's lab developed transgenic mice overexpressing the SK3 gene (OHSU Tech ID #515). In this invention, a new mouse model was developed which maintains wild-type expression levels while allowing for site-specific deletion of the SK3 gene. Deletion of the resulting SK3 protein can either be region specific or the entire germline. These animals can be used to test the physiological role of the SK3 channel by using region-specific or cell-type specific deletions.
Phylogenetically older brain regions such as the thalamus, basal ganglia, cerebellum, and brainstem show high levels of SK3 gene expression. SK3 channels represent potential therapeutic targets for many disorders. Some examples of disorders in which SK3 channels play a role include:
· sleep apnea or sudden infant death syndrome
· dopamine related disorders (Parkinson's disease, etc.)
· bipolar disorder
· memory loss associated with aging
· breast cancer cell migration
· urinary bladder infections
· anorexia nervosa
· high blood pressure
SK3 channels also are potentially involved with regulating uterine contractions during labor. Furthermore, SK3 is the only known SK subunit expressed in skeletal muscle where expression is highly induced by denervation and in primary cultured myotubes.
No other such mice are known to exist.
John Adelman received his Ph.D. in Microbiology from Oregon Health & Science University in 1988. He holds a B.S. and an M.S. in Microbiology from the University of Connecticut. After a year as a research assistant at Yale University, he spent five years as a research associate at Genentech. He arrived at the Vollum Institute in 1985, where he did his graduate research. After receiving his Ph.D., he accepted a faculty position at the Vollum and was promoted to senior scientist in 1998. Adelman holds concurrent appointments in the Departments of Cell and Developmental Biology and Molecular and Medical Genetics in the School of Medicine.
Dr. Adelman's lab continues to utilize these mice to study the role of SK3.
- John Adelman, VI.Vollum Institute
- Chris Bond, VI.Vollum Institute
- James Maylie, SM.Obstetrics & Gynecology
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Technology Development Manager