Small-Conductance CA2+-Activated Potassium Channel 2 (SK2) Transgenic Mice
OHSU # 0891 B
- John Adelman, VI.Vollum Institute
- Chris Bond, VI.Vollum Institute
- James Maylie, SM.Obstetrics & Gynecology
In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential. Three SK channel subunits have been molecularly characterized. These channels are found in many types of neurons as well as in some other cell types.
In this invention, homologous recombination was used to floxed the SK2 gene in mouse ES cells. Subsequent routine manipulations generated homozygous floxed mice. These have been crossed to a series of Cre mice that generate either global or tissue delimited null SK2 mice.
Most of the higher brain regions such as the neocortex and hippocampus show expression of genes encoding SK2 channels. SK2 mRNA is observed in some regions such as the thalamus and in the reticular formation, but not in monoaminergic systems. High levels of SK2 has been observed in the liver and heart. These mice could be used to test potential therapies for disorders in which SK2 channels are implicated.
John Adelman received his Ph.D. in Microbiology from Oregon Health & Science University in 1988. He holds a B.S. and an M.S. in Microbiology from the University of Connecticut. After a year as a research assistant at Yale University, he spent five years as a research associate at Genentech. He arrived at the Vollum Institute in 1985, where he did his graduate research. After receiving his Ph.D., he accepted a faculty position at the Vollum and was promoted to senior scientist in 1998. Adelman holds concurrent appointments in the Departments of Cell and Developmental Biology and Molecular and Medical Genetics in the School of Medicine.
James Maylie received his Ph.D. from Oregon Health & Science University in 1977. He is a Professor in the Department of Obstetrics and Gynecology, School of Medicine at Oregon Health & Science University.
Dr. Adelman’s lab continues to utilize these mice to study the role of SK2.
J Neurosci. 2004 Jun 9;24(23):5301-6: Small conductance Ca2+-activated K+ channel knock-out mice reveal the identity of calcium-dependent after hyperpolarization currents.
- OHSU # 0891 — Small-Conductance CA2+-Activated Potassium Channel 1 (SK1) Transgenic Mice
- OHSU # 0515 — Small-Conductance Ca2+-Activated Potassium Channel 3 (SK3) transgenic mice
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