Categories | Inventors

CBP Expression Vector

OHSU # 0536

Categories:

• Biological Materials - Protein/Peptides
• Biological Materials

Inventors:

• Richard Goodman, VI.Vollum Institute

Summary:

Technology Overview

Cyclic AMP-regulated gene expression frequently involves a DNA element known as the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. This modification stimulates interaction with one or more of the general transcription factors or, alternatively, allows recruitment of a co-activator. CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of M(r) 265K which we term CBP (for CREB-binding protein).

Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric protein to function as a protein kinase A-regulated transcriptional activator. We propose that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB.

For more information, contact:

Robert Copenhaver
Technology Development Manager
503-494-7809