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Global profiling of protein synthesis in genetically targeted cell populations

 

OHSU # 1945

Technology Overview:

While techniques exist for purifying ribosome-associated mRNAs in specific cell types and labeling nascent proteins in flow cytometry-sorted cells, currently available methods do not permit cell-specific labeling and purification of nascent proteomes in vivo from heterogeneous tissues. To accomplish this, Dr. Cohen and his team have developed a novel chemical genetic strategy that will enable us, for the first time, to globally profile new protein synthesis in a cell-type specific manner in genetically targeted cell populations. This novel strategy circumvents the need for laborious cell purification as the tissues can simply be homogenized and the small molecule-labeled proteins purified and enriched by affinity chromatography. This unique chemical genetic strategy has the potential to provide unprecedented insight into translational regulation in animals.

While techniques exist for purifying ribosome-associated mRNAs in specific cell types and labeling nascent proteins in flow cytometry-sorted cells, currently available methods do not permit cell-specific labeling and purification of nascent proteomes in vivo from heterogeneous tissues. To accomplish this, Dr. Cohen and his team have developed a novel chemical genetic strategy that will enable us, for the first time, to globally profile new protein synthesis in a cell-type specific manner in genetically targeted cell populations. This novel strategy circumvents the need for laborious cell purification as the tissues can simply be homogenized and the small molecule-labeled proteins purified and enriched by affinity chromatography. This unique chemical genetic strategy has the potential to provide unprecedented insight into translational regulation in animals.

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Travis Cook
Senior Technology Development Manager
503-494-3229