Global profiling of protein synthesis in genetically targeted cell populations
OHSU # 1945
While techniques exist for purifying ribosome-associated mRNAs in specific cell types and labeling nascent proteins in flow cytometry-sorted cells, currently available methods do not permit cell-specific labeling and purification of nascent proteomes in vivo from heterogeneous tissues. To accomplish this, Dr. Cohen and his team have developed a novel chemical genetic strategy that will enable us, for the first time, to globally profile new protein synthesis in a cell-type specific manner in genetically targeted cell populations. This novel strategy circumvents the need for laborious cell purification as the tissues can simply be homogenized and the small molecule-labeled proteins purified and enriched by affinity chromatography. This unique chemical genetic strategy has the potential to provide unprecedented insight into translational regulation in animals.
- Michael Cohen, SM.Physiology & Pharmacology
- Ruth Barrett, VI.Vollum Institute
- Haihong Jin, SM.Physiology & Pharmacology
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