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Split-luciferase complementation to measure soluble epoxide hydrolase dimerization

 

OHSU # 1803

Summary:

Novel assay and technique to monitor the effect of mutations on dimerization. The enzyme soluble epoxide hydrolase (sEH) forms a catalytically active dimer with predominantly cytosolic localization. Disrupting dimerization, on the other hand, reduces sEH hydrolase activity and shifts the sub-cellular localization of sEH to peroxisomes. The peroxisomal localization of sEH may contribute to the protective role in a number of different diseases such as ischemia. To identify small molecular inhibitors of sEH dimerization, a novel assay was developed using a split-firefly luciferase strategy.

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Michele Gunness
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