OHSU

Southern blot analysis at OHSU

Definition
Southern blot analysis is a an assay used in molecular biology to assert the presence/absence status of a specific DNA sequence in DNA samples. DNA samples to be assayed are first digested by restriction enzymes, fragments are then resolved by gel electrophoresis following by a blotting ensuring transfer to nitrocellulose or nylon membrane. Immobilization of DNA fragments to the membrane is achieved by UV crosslinking and/or baking. Probes raised against the specific sequences are then hybridized to the membrane. Detection of hybridization signals is carried out by immunofluorescence or radioactivity measurements using photographic films or digital imaging devices such as Phosphor Imager.
Synonyms
Southern blot
Categories
→ Southern blot analysis
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Molecular Virology Support Core

Division of Pathobiology & Immunology, Contact: Kahl, Christoph, Ph.D

  • Southern blot for viral DNA