OHSU

Reversible terminator sequencing at OHSU

Definition
is a DNA sequencing which allows sequence identification by relying on use of DNA polymerase and reversible terminator. The methods requires immobilization of genomic DNA fragment onto a surface and a specific clonal amplification step known as bridge PCR. Reliance on reversible terminator allow cycles of DNA chain extension by DNA polymerase and imaging without the need of electrophoretic separation of newly synthesized DNA fragment as with Sanger sequencing.
Synonyms
Solexa sequencing
Categories
→ Reversible terminator sequencing
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Gene Profiling Shared Resource Core Laboratory

Department of Behavioral Neuroscience, Contact: Harrington, Chris, Ph.D.

  • Copy number variation analysis