OHSU

Restriction fragment length polymorphism electrophoresis at OHSU

Definition
A nucleic acid assay that is used to detect RFLPs, which involves fragmenting a sample of DNA by a restriction enzyme. The resulting DNA fragments are then separated by length using agarose gel electrophoresis, and transferred to a membrane via the Southern blot procedure. Hybridization of the membrane to a labeled DNA probe then determines the length of the fragments which are complementary to the probe. A RFLP occurs when the length of a detected fragment varies between individuals.
Synonyms
RFLP electrophoresis
Categories
→ Restriction fragment length polymorphism electrophoresis
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DNA Sequence Analysis Shared Resource

Contact: Keller, Thomas J., PhD

  • RFLP genotyping service