OHSU

PCR at OHSU

Definition
PCR is the process in which a DNA polymerase is used to amplify a piece of DNA by in vitro enzymatic replication. As PCR progresses, the DNA thus generated is itself used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified.
Synonyms
Polymerase chain reaction
Subcategories
Amplified fragment length polymorphism PCR, Nested polymerase chain reaction, Real-time PCR
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DNA Sequence Analysis Shared Resource

Contact: Keller, Thomas J., PhD

  • ABI 2720 thermal cycler
  • ABI GeneAmp 9700 96-well PCR system
 

Gene Profiling Shared Resource Core Laboratory

Department of Behavioral Neuroscience, Contact: Harrington, Chris, Ph.D.

  • MJ Research PTC-225 Tetrad thermal cycler
  • Veriti Thermal Cylcer
  • Basic data analysis
 

Histopathology Shared Resource

Knight Cancer Institute, Contact: Thomas, George, M.D.

  • ABI GeneAmp 9700 96-well PCR system
 

Molecular Virology Support Core

Division of Pathobiology & Immunology, Contact: Kahl, Christoph, Ph.D

  • ABI GeneAmp 9700 96-well PCR system
  • PCR hood
  • Real time PCR titer of lentivirus prep with sample prep
 

Oregon Clinical & Translational Research Institute Core Laboratory

Oregon Clinical & Translational Research Institute, Contact: Clemons, Aaron, B.Sc.

  • ABI 9800 Fast thermal cycler
  • ABI GeneAmp 9700 96-well PCR system
  • MJ Research Tetrad 4 block PCR machine