OHSU

Nucleic acid microarray assay at OHSU

Definition
A nucleic acid assay used to measure changes in gene expression levels, to detect single nucleotide polymorphisms (SNPs), or to genotype or resequence mutant genomes. It consists of an arrayed series of thousands of microscopic spots of DNA oligonucleotides, called features, each containing picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters). These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA sample (called target) under high-stringency conditions. Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target. Since an array can contain tens of thousands of probes, a microarray experiment can accomplish many genetic tests in parallel.
Categories
→ Nucleic acid microarray assay
Subcategories
Affymetrix array
See also: Affymetrix array
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Gene Profiling Shared Resource Core Laboratory

Department of Behavioral Neuroscience, Contact: Harrington, Chris, Ph.D.

  • Agilent 2100 Bioanalyzer
  • Illumina BeadArray
  • Affymetrix GeneChip Command Console
  • Affymetrix Microarray Suite
  • GeneSifter