OHSU

Next generation sequencing of target genes at OHSU

Definition
A DNA sequencing technique that became commercially available in 2004 and is used by specific commercial platforms that embody a complex interplay of enzymology, chemistry, high-resolution optics, hardware, and software engineering. These instruments allow highly streamlined sample preparation steps prior to DNA sequencing, which provides a significant time savings and a minimal requirement for associated equipment in comparison to the highly automated, multistep pipelines necessary for clone-based high-throughput sequencing. Each technology amplifies single strands of a fragment library and perform sequencing reactions on the amplified strands. The fragment libraries are obtained by annealing platform-specific linkers to blunt-ended fragments generated directly from a genome or DNA source of interest. Because the presence of adapter sequences means that the molecules then can be selectively amplified by PCR, and no bacterial cloning step is required to amplify the genomic fragment in a bacterial intermediate as is done in traditional sequencing approaches.
Synonyms
Next generation sequencing, Genome sequencing, High throughput DNA sequencing, High throughput nucleotide sequencing, NGS, Next gen, Next gen sequencing, Nucleotide sequencing, high-throughput, Second generation sequencing, Sequencing, high-throughput nucleotide
Categories
→ Next generation sequencing of target genes
Subcategories
Massively parallel signature sequencing, Solexa sequencing
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Gene Profiling Shared Resource Core Laboratory

Department of Behavioral Neuroscience, Contact: Harrington, Chris, Ph.D.

  • Ion Torrent PGM Sequencer
  • RNA sequencing service
  • Small genome focused panels on Ion Torrent PGM
 

Massively Parallel Sequencing Shared Resource

Contact: Carlos, Amy

  • De novo sequencing for unique genomes
  • DNA resequencing for copy number variation discovery
  • DNA resequencing for single nucleotide polymorphism discovery
  • Casava
  • SeqMonk