Amplified fragment length polymorphism PCR at OHSU
- A PCR based technique used to detect polymorphisms in DNA. It uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments are then selected to be amplified and the amplified fragments are visualized on denaturing polyacrylamide gels either through autoradiography or fluorescence methodologies.
- AFLP, AFLP-PCR
- Polymerase chain reaction → Amplified fragment length polymorphism PCR
Contact: Keller, Thomas J., PhD
- Nucleic acid fragment analysis