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Amplified fragment length polymorphism PCR at OHSU

Definition
A PCR based technique used to detect polymorphisms in DNA. It uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments are then selected to be amplified and the amplified fragments are visualized on denaturing polyacrylamide gels either through autoradiography or fluorescence methodologies.
Synonyms
AFLP, AFLP-PCR
Categories
→ Amplified fragment length polymorphism PCR
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DNA Sequence Analysis Shared Resource

Contact: Keller, Thomas J., PhD

  • Nucleic acid fragment analysis