OHSU

AFLP at OHSU

Definition
A PCR based technique used to detect polymorphisms in DNA. It uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments. A subset of the restriction fragments are then selected to be amplified and the amplified fragments are visualized on denaturing polyacrylamide gels either through autoradiography or fluorescence methodologies.
Synonyms
Amplified fragment length polymorphism PCR, AFLP-PCR
Categories
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DNA Sequence Analysis Shared Resource

Contact: Keller, Thomas J., PhD

  • Nucleic acid fragment analysis