Oligo Synthesis Update

  • siRNA We can synthesize & prepare duplex siRNA for $180, or individual strands for $80 a piece. Minimum order: 8 siRNA oligos (or 4 pairs).
  • Modified oligos and labels, the cost of the reagent is the only additional charge
  • Longmers with good yield out to 150 nucleotides.
  • Most primers are ready the next morning.
    mark as "rush" to go to the head of the queue.

Did you know?

Synthetic oligos are single stranded with hydroxyl groups (not phosphates) at both termini. To form a duplex with the complement: simply add equimolar amounts to the your buffered solution, heat to well over the Tm, then allow to slowly cool to room temperature on the bench. Store cold.

Synthesis Services

  • Primer scale (40 nmole) deoxyoligo: $1/base plus process fee ($10 standard, desalted)
  • Large scale (200 nmole) deoxyoligo: $2/base plus process fee ($10 standard, desalted)
  • Venti scale (1000 nmole) deoxyoligo: $5/base plus process fee ($10 standard, desalted)
  • siRNA: $1/base plus process fee ($59 standard, desalted)
  • The only extra charge for labels, e.g. fluorescent dyes, and other modifications (e.g., biotin, phosphate, quenchers) is the cost of the special reagent.

The most convenient way to order oligos is the on-line work request. Email the Core Facility for more information.

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FAQ

Standard synthesis products are purified on a sephadex column and quantified by UV absorbance. Note, that there is a hydroxyl (OH) on both termini.

Labels and other modifications

We can incorporate anything available as a reagent for an automated synthesizer. No additional charge besides the cost of the reagent. No additional charge for mixed base (degenerate) oligos either.

    Examples

  • Fluoresecent labels, both 5' and internal: Cyanine dyes, Fluorescein, Epoch "Black Hole" quencher dyes, etc.
  • 2'-OMe and 2'-F -RNA
  • Linked-nucleic-acid probes with higher Tm for improved mismatch discrimination
  • on-machine esterification (coupling rx) of any NHS-labels amenable to CEP-solid-phase synthesis cycles using FMOC-amino-modifier C6-dT

Scheduling

FIFO, but most are ready the next day. If you need them in the morning, mark them as "Rush".

Links, Notes and Methods

The most convenient way to order oligos is the on-line work request. Email the Core Facility for more information.

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