Microscopy Image Gallery

Wide-Field Epifluorescence: Before and After Deconvolution

Image Restoration on the Applied Precision CoreDV

Cho cells stained for DNA (blue), ASIC2-gfp and BIP (red), Dr. Sunghee Chai, Sinon Lab (Legacy Research)

Image Restoration of “Z stack”
wide-field epi-fluorescence

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Images are acquired at specified focal distances from the top of the selected area to the bottom in each color specified sequentially. The colors are merged to display the information for each Z which can then also be viewed “in motion” by moving through the entire stack with a mouse control. This specimen of CHO cells, ASIC-gfp fixed and stained for mitochondria and DNA. provided by Dr. Sunghee Chai, Sinon Lab (Legacy).

Image Restoration of confocal image: Drosophila embryo cells

Drosophila embryo cells in synchronous division during development for the Smolik Lab (OHSU).

Fluourescence Recovery After Photo-bleaching

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Image shows a reduction of tubulin-gfp fluorescent intensity after exposure to a 488 laser and the subsequent recovery in time. This is an example of a liquid state of the protein to which the GFP is attached: recovery is fairly rapid. Vero cells were provided by the Johnson lab (OHSU) and transiently transfected with a tubulin motor construct, EB1-gfp.

Plot of FRAP

The background measurement was taken outside the cell; the control (Cont) was taken from the other side of the cell and the FRAP from the laser target.

Image Restoration Movie: Calcium Imaging

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Images of Fura 2 calcium indicator in Mouse T cells synapsing with mouse fibroblasts stably transfected with MHC II -gfp. The calcium flux happens at the first contact of the T cell with the fibroblast and lasts more than 1 hour. The MHC II is not very visible until the T cell connects and then rapidly aggregates at the site of the synpase, seen as the bright green line between the two types of cells. For the Parker Lab (OHSU).

Cell Surface Activity, Wide-field, not TIRF

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Normal excitation with wide-field illumination which collects fluorescence from molecules above and below the focal plane, seen as a general haze in the background. Compare with the following TIRF image. Johnson lab (OHSU)

Cell Surface Activity, with TIRF

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Only the tubulin motor EB1-gfp chimeric molecules close enough to the coverglass are in focus (brighter). Compare with the previous movie taken with normal wide-field excitation. Johnson lab (OHSU)

Cell Surface Activity, with TIRF combined with DIC

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Combined TIRF & DIC over time. Johnson lab (OHSU)