NIH Requests Acknowledgement
For each publication that results from grant support provided by the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), grantees must include an acknowledgment of support and a disclaimer stating the following:
Option 1
This publication was made possible by Grant Number S10-RR023432 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH.
Option 2
The project described was supported by Grant Number S10-RR023432 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) and its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH.
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Corelab Acknowledgement (boilerplate)
for work on the new Deltavision RT image restoration system
The Core DV system is a high resolution wide field microscope for acquiring images of live or fixed cells and tissues. The basic system is based on the Olympus IX71 microscope with differential interference contrast (DIC) transmitted light and 3 lasers (402nm, 488nm, 532nm) for photoactivation (PA), photobleaching (FRAP) and total internal reflection (TIRF). The light source is a short arc Xenon lamp and the excitation filter wheel has 10 places while the emission has 6. Both wheels are exchangeable for fluorescent protein-specific or user-specific filters. More info about standard and live cell filter sets here. The camera is a Nikon Coolpix HQ for fast, sensitive acquisition. The patented stage is controlled by extremely accurate XYZ nanomotors for exact z-stack and point-visiting functions.
for work on the old Deltavision Instrumentation
The Applied Precision DeltaVision™ "Image Restoration System" includes a chassis with precision motorized XYZ stage, a Nikon TE200 inverted fluorescent microscope, halogen illumination with API light homogenizer, a CH350L camera (500 KHz, 12-bit, 2 Mp, KAF 1400 GL, 1317x1035, liquid cooled), DeltaVision software, custom optical filters, time-lapse capabilities. Deconvolution using the iterative constrained algorithm of Sedat and Agard and additional image processing is performed on an SGI Octane workstation.
for the BioRad Confocal at the OHRC
We perform confocal microscopy on the Oregon Hearing Research Center's new OHRC Olympus confocal which is the Fluoview-1000 (FV1000) on an IX-81 microscope base; an electronic turret with 10x and 20x air and 40x and 60x oil objectives. It has 405, 440, 488, 515 and 559 nm excitation laser lines and can simultaneously image while initiating a laser event such as uncaging, FRAP, FLIP, and photoactivation. It can acquire a differential interference contrast (DIC) and overlay it with the fluorescence images.